Human GATA-1 PE-conjugated Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human GATA-1 in direct ELISAs. In Western blots, this antibody does not cross-react with recombinant human (rh) GATA-2 or rhGATA-6.
  • Source
    Monoclonal Rat IgG2B Clone # 234732
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human GATA-1
    Met1-Ser413
    Accession # P15976
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry

Detection of GATA‑1 in MG‑63 Human Cell Line by Flow Cytometry. MG‑63 human osteosarcoma cell line was stained with Rat Anti-Human GATA‑1 PE‑conjugated Monoclonal Antibody (Catalog # IC1779P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GATA-1

GATA-1 is the founding member of the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue‑specific programs of gene expression and morphogenesis. GATA-1 is expressed in blood forming cells. It interacts with several additional proteins to activate or repress gene expression and is essential for erythropoiesis (1).

  • References:
    1. Patient, R.K. and J. McGhee (2002) Curr. Opin. Genet. Dev. 12:416.
  • Entrez Gene IDs:
    2623 (Human); 14460 (Mouse)
  • Alternate Names:
    Eryf1; ERYF1GATA-binding protein 1 (globin transcription factor 1); GATA binding protein 1 (globin transcription factor 1); GATA1; GATA-1; GATA-1erythroid transcription factor; GATA-binding factor 1; GF-1; GF1globin transcription factor 1; NF-E1 DNA-binding protein; NFE1erythroid transcription factor 1; transcription factor GATA1; XLTT
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human GATA-1 PE-conjugated Antibody IC1779P
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 Flow Human Anonymous 03/27/2017
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Flow Cytometry Human GATA-1 PE-conjugated Antibody IC1779P
Flow Cytometry: Human GATA-1 PE-conjugated Antibody [IC1779P]

Summary

ApplicationFlow Cytometry
Sample TestedMCF-7 human breast cancer cell line
SpeciesHuman

Other Experimental Details

Other Experimental DetailsIntracellular detection of GATA-1 in MCF-7 cells using the PE-conjugated Human GATA-1 antibody (#IC1779P, orange) at 10 uL/10^6 cells, or Isotype Control antibody (black). Cells were fixed with 2% PFA for 10 min and permeabilized with 0.2% Tween-20 for 20 min prior to staining. Staining buffer: 0.2% Tween-20.

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