Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 234732
View all GATA-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-1
Met1-Ser413
Accession # P15976

Specificity

Detects human GATA-1 in direct ELISAs. In Western blots, this antibody does not cross-react with recombinant human (rh) GATA-2 or rhGATA-6.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human GATA‑1 PE‑conjugated Antibody

Detection of GATA-1 antibody in MG-63 Human Cell Line antibody by Flow Cytometry.

Detection of GATA‑1 in MG‑63 Human Cell Line by Flow Cytometry.

MG-63 human osteosarcoma cell line was stained with Rat Anti-Human GATA-1 PE-conjugated Monoclonal Antibody (Catalog # IC1779P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human GATA‑1 PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: MG‑63 human osteosarcoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 1 review rated 5 using IC1779P in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: GATA-1

GATA-1 is the founding member of the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue‑specific programs of gene expression and morphogenesis. GATA-1 is expressed in blood forming cells. It interacts with several additional proteins to activate or repress gene expression and is essential for erythropoiesis (1).

References

  1. Patient, R.K. and J. McGhee (2002) Curr. Opin. Genet. Dev. 12:416.

Alternate Names

GATA1

Entrez Gene IDs

2623 (Human); 14460 (Mouse)

Gene Symbol

GATA1

UniProt

P15976

Additional GATA-1 Products

Product Documents for Human GATA‑1 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GATA‑1 PE‑conjugated Antibody

For research use only

Customer Reviews for Human GATA‑1 PE‑conjugated Antibody (1)

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  • Human GATA-1 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: MCF-7 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 03/27/2017
    Intracellular detection of GATA-1 in MCF-7 cells using the PE-conjugated Human GATA-1 antibody (#IC1779P, orange) at 10 uL/10^6 cells, or Isotype Control antibody (black). Cells were fixed with 2% PFA for 10 min and permeabilized with 0.2% Tween-20 for 20 min prior to staining. Staining buffer: 0.2% Tween-20.
    Human GATA‑1 PE‑conjugated Antibody IC1779P

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