Intracellular Staining by Flow Cytometry
Detection of GATA‑1 in MG‑63 Human Cell Line by Flow Cytometry.MG‑63 human osteosarcoma cell line was stained with Rat Anti-Human GATA‑1 PE‑conjugated Monoclonal Antibody (Catalog # IC1779P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
GATA-1 is the founding member of the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue‑specific programs of gene expression and morphogenesis. GATA-1 is expressed in blood forming cells. It interacts with several additional proteins to activate or repress gene expression and is essential for erythropoiesis (1).
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Flow Cytometry: Human GATA-1 PE-conjugated Antibody [IC1779P]
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