Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 234732
View all GATA-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GATA-1
Met1-Ser413
Accession # P15976

Specificity

Detects human GATA-1 in direct ELISAs. In Western blots, this antibody does not cross-react with rhGATA-2 or rhGATA-6.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Human GATA‑1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion-fixed MG-63 human osteosarcoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human GATA‑1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GATA-1

GATA-1 is the founding member of the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue‑specific programs of gene expression and morphogenesis. GATA-1 is expressed in blood forming cells. It interacts with several additional proteins to activate or repress gene expression and is essential for erythropoiesis (1).

References

  1. Patient, R.K. and J. McGhee (2002) Curr. Opin. Genet. Dev. 12:416.

Alternate Names

GATA1

Entrez Gene IDs

2623 (Human); 14460 (Mouse)

Gene Symbol

GATA1

UniProt

P15976

Additional GATA-1 Products

Product Documents for Human GATA‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GATA‑1 Antibody

For research use only

Citations for Human GATA‑1 Antibody

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Protocols

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FAQs

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