Human GBX2 Antibody

AF4638 1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4638
AF4638-SP

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Detection of Human GBX2 by Western Blot.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GBX2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GBX2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GBX2
Lys126-Asn247
Accession # P52951

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot

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Detection of Human GBX2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human GBX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GBX2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GBX2

GBX2 (gastrulation brain homeobox) is a member of the homeobox family, which shares a 180 bp DNA sequence that encodes the DNA binding homeodomain. GBX2, along with OTX2 and FGF8 establish midbrain-hindbrain boundries in the developing brain. Increased GBX2 expression has also been associated with pluripotent cells and metastasic potential in cancers.

Long Name

Gastrulation Brain Homeobox 2

Entrez Gene IDs

2637 (Human); 14472 (Mouse); 114500 (Rat)

Alternate Names

Gastrulation and brain-specific homeobox protein 2; gastrulation brain homeo box 2; gastrulation brain homeobox 2; GBX2; homeobox protein GBX-2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human GBX2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Generation of thalamic neurons from mouse embryonic stem cells
Authors: A Shiraishi, K Muguruma, Y Sasai
Development, 2017;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC