Human GBX2 Antibody

R&D Systems | Catalog # AF4638

R&D Systems
Discontinued Product
AF4638 has been discontinued. View all GBX2 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GBX2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GBX2
Lys126-Asn247
Accession # P52951

Specificity

Detects human GBX2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human GBX2 Antibody

Detection of Human GBX2 antibody by Western Blot.

Detection of Human GBX2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human GBX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GBX2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human GBX2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and U937 human histiocytic lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GBX2

GBX2 (gastrulation brain homeobox) is a member of the homeobox family, which shares a 180 bp DNA sequence that encodes the DNA binding homeodomain. GBX2, along with OTX2 and FGF8 establish midbrain-hindbrain boundries in the developing brain. Increased GBX2 expression has also been associated with pluripotent cells and metastasic potential in cancers.

Long Name

Gastrulation Brain Homeobox 2

Alternate Names

Gastrulation and brain-specific homeobox protein 2, gastrulation brain homeo box 2, gastrulation brain homeobox 2, homeobox protein GBX-2

Entrez Gene IDs

2637 (Human); 14472 (Mouse); 114500 (Rat)

Gene Symbol

GBX2

UniProt

P52951

Additional GBX2 Products

Product Documents for Human GBX2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GBX2 Antibody

For research use only

Citations for Human GBX2 Antibody

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