Human GBX2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human GBX2 by Western Blot.
1 Image
Product Details
Citations (1)
Supplemental Products

Human GBX2 Antibody Summary

Species Reactivity
Detects human GBX2 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human GBX2
Accession # P52951
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human GBX2 antibody by Western Blot. View Larger

Detection of Human GBX2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human GBX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GBX2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GBX2

GBX2 (gastrulation brain homeobox) is a member of the homeobox family, which shares a 180 bp DNA sequence that encodes the DNA binding homeodomain. GBX2, along with OTX2 and FGF8 establish midbrain-hindbrain boundries in the developing brain. Increased GBX2 expression has also been associated with pluripotent cells and metastasic potential in cancers.

Long Name
Gastrulation Brain Homeobox 2
Entrez Gene IDs
2637 (Human); 14472 (Mouse); 114500 (Rat)
Alternate Names
Gastrulation and brain-specific homeobox protein 2; gastrulation brain homeo box 2; gastrulation brain homeobox 2; GBX2; homeobox protein GBX-2

Product Datasheets

Citation for Human GBX2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Generation of thalamic neurons from mouse embryonic stem cells
    Authors: A Shiraishi, K Muguruma, Y Sasai
    Development, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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