Human Glut1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1418P
Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (6)
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Human Glut1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.
Source
Monoclonal Mouse IgG2B Clone # 202915
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Glut1
Met1-Val492
Accession # AAA52571
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Glut1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 PE-conjugated Monoclonal Antibody (Catalog # FAB1418P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Glut1 antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Glut1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line either (A) untreated or (B) cultured in nutrient-depleted media was stained with Mouse Anti-Human Glut1 PE-conjugated Monoclonal Antibody (Catalog # FAB1418P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glut1

Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8+ T cells, and erythrocytes (1‑4). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.

References
  1. Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
  2. Meuckler, M. et al. 1985, Science 229:941.
  3. Jones, K.S. et al. 2006, J. Virol. 8291.
  4. Takenouchi, N. et al. 2007, J. Virol. 1506.
  5. Kinet, S. et al. 2007, Retrovirology 4:31.
Long Name
Glucose Transporter Type 1
Entrez Gene IDs
6513 (Human); 20525 (Mouse)
Alternate Names
Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis); CSE; DYT17; DYT18; DYT9; EIG12; Glucose transporter type 1, erythrocyte/brain; Glut1; GLUT-1; GLUT1DS; HepG2 glucose transporter; HTLVR; Human T-Cell Leukemia Virus (I and II) Receptor; MGC141895; MGC141896; PED; SLC2A1; solute carrier family 2 (facilitated glucose transporter), member 1; Solute Carrier Family 2 Member 1; solute carrier family 2, facilitated glucose transporter member 1

Product Datasheets

Citations for Human Glut1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Impact of acute exercise on peripheral blood mononuclear cells nutrient sensing and mitochondrial oxidative capacity in healthy young adults
    Authors: B Theall, J Stampley, E Cho, J Granger, NM Johannsen, BA Irving, G Spielmann
    Physiological Reports, 2021;9(23):e15147.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Host-Derived Lipids from Tuberculous Pleurisy Impair Macrophage Microbicidal-Associated Metabolic Activity
    Authors: JL Marín Fran, M Genoula, D Corral, G Duette, M Ferreyra, M Maio, MB Dolotowicz, OE Aparicio-T, E Patiño-Mar, A Charton, A Métais, F Fuentes, V Soldan, EJ Moraña, D Palmero, M Ostrowski, P Schierloh, C Sánchez-To, R Hernández-, J Pedraza-Ch, Y Rombouts, D Hudrisier, E Layre, C Vérollet, I Maridonnea, O Neyrolles, MDC Sasiain, G Lugo-Villa, L Balboa
    Cell Reports, 2020;33(13):108547.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Monocyte glycolysis determines CD8+ T cell functionality in human Chagas disease
    Authors: LM Sanmarco, N Eberhardt, G Bergero, LP Quebrada P, PM Adami, LM Visconti, ÁR Minguez, Y Hernández-, EAC Silva, L Morelli, M Postan, MP Aoki
    JCI Insight, 2019;4(18):.
    Species: Human
    Sample Types: blood
    Applications: Flow Cytometry
  4. IL-7 Restores T Lymphocyte Immunometabolic Failure in Septic Shock Patients through mTOR Activation
    Authors: F Venet, J Demaret, BJ Blaise, C Rouget, T Girardot, E Idealisoa, T Rimmelé, F Mallet, A Lepape, J Textoris, G Monneret
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Erosion of the chronic myeloid leukaemia stem cell pool by PPARgamma agonists.
    Authors: Prost S, Relouzat F, Spentchian M, Ouzegdouh Y, Saliba J, Massonnet G, Beressi J, Verhoeyen E, Raggueneau V, Maneglier B, Castaigne S, Chomienne C, Chretien S, Rousselot P, Leboulch P
    Nature, 2015;525(7569):380-3.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. WASH knockout T cells demonstrate defective receptor trafficking, proliferation, and effector function.
    Authors: Piotrowski, Joshua T, Gomez, Timothy, Schoon, Renee A, Mangalam, Ashutosh, Billadeau, Daniel D
    Mol Cell Biol, 2013;33(5):958-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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