Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although
Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.
Monoclonal Mouse IgG2B Clone # 202915
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with human Glut1 Met1-Val492 Accession # AAA52571
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 700
5 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB1418N, filled histogram) or isotype control antibody (Catalog # IC0041N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8+ T cells, and erythrocytes (1‑4). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.
Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
Meuckler, M. et al. 1985, Science 229:941.
Jones, K.S. et al. 2006, J. Virol. 8291.
Takenouchi, N. et al. 2007, J. Virol. 1506.
Kinet, S. et al. 2007, Retrovirology 4:31.
Glucose Transporter Type 1
Entrez Gene IDs:
6513 (Human); 20525 (Mouse)
DYT17; DYT18; DYT18DYT17; DYT9; EIG12; Glucose transporter type 1, erythrocyte/brain; GLUT-1; GLUT1DS; GLUT1GLUT; HepG2 glucose transporter; MGC141895; MGC141896; PED; SLC2A1; solute carrier family 2 (facilitated glucose transporter), member 1; solute carrier family 2, facilitated glucose transporter member 1
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.