Human Glut1 Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1418N
Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry.
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Citations (2)
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Human Glut1 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glut1. Stains human Glut1-transfected NS0 cells, but not NS0 control transfectants. Although Human Glut1 Antibody detects Glut1 on the surface of T cells (1, 2), it does not detect it on erythrocytes (5). The reason for this discrepancy is not understood, but may be related to conformational or post-translational modification differences.
Source
Monoclonal Mouse IgG2B Clone # 202915
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Glut1
Met1-Val492
Accession # AAA52571
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Glut1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Glut1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut1 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB1418N, filled histogram) or isotype control antibody (Catalog # IC0041N, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glut1

Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8+ T cells, and erythrocytes (1‑4). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.

References
  1. Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
  2. Meuckler, M. et al. 1985, Science 229:941.
  3. Jones, K.S. et al. 2006, J. Virol. 8291.
  4. Takenouchi, N. et al. 2007, J. Virol. 1506.
  5. Kinet, S. et al. 2007, Retrovirology 4:31.
Long Name
Glucose Transporter Type 1
Entrez Gene IDs
6513 (Human); 20525 (Mouse)
Alternate Names
Choreoathetosis/Spasticity, Episodic (Paroxysmal Choreoathetosis); CSE; DYT17; DYT18; DYT9; EIG12; Glucose transporter type 1, erythrocyte/brain; Glut1; GLUT-1; GLUT1DS; HepG2 glucose transporter; HTLVR; Human T-Cell Leukemia Virus (I and II) Receptor; MGC141895; MGC141896; PED; SLC2A1; solute carrier family 2 (facilitated glucose transporter), member 1; Solute Carrier Family 2 Member 1; solute carrier family 2, facilitated glucose transporter member 1

Product Datasheets

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human Glut1 Alexa Fluor® 700-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. A novel human induced pluripotent stem cell blood-brain barrier model: Applicability to study antibody-triggered receptor-mediated transcytosis
    Authors: M Ribecco-Lu, C Sodja, J Haukenfrer, AS Haqqani, D Ly, P Zachar, E Baumann, M Ball, J Huang, M Rukhlova, M Martina, Q Liu, D Stanimirov, A Jezierski, M Bani-Yagho
    Sci Rep, 2018;8(1):1873.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. IL-1? induced HIF-1? inhibits the differentiation of human FOXP3(+) T cells
    Authors: LM Feldhoff, CM Rueda, ME Moreno-Fer, J Sauer, CM Jackson, CA Chougnet, J Rupp
    Sci Rep, 2017;7(1):465.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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