Human Glut5 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1349R-025
Detection  of Glut5 in NS0 Mouse Cell Line Transfected with Human Glut5 by Flow  Cytometry.
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Human Glut5 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glut5. Stains human Glut5 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 195205
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Glut5
Met1-Gln501
Accession # P22732
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of Glut5 in NS0 Mouse Cell Line Transfected with Human Glut5 by Flow  Cytometry. View Larger

Detection of Glut5 in NS0 Mouse Cell Line Transfected with Human Glut5 by Flow Cytometry. NS0 mouse myeloma cell line transfected with either human Glut5 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human Glut5 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB1349R). View our protocol for Staining Membrane-associated Proteins.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glut5

Glut5 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues. Glut5 is expressed at highest levels in small intestine and at lower levels in kidney, testis, skeletal muscle, and adipose tissue (1, 2). Glut5 transports fructose in intestine, testis, and other tissues.

References
  1. Kayano, T. et al. (1990) J. Biol. Chem. 265:13276.
  2. Corpe, C.P. (2002) Biochem. Biophys. Acta. 1576:191.
  3. Sasaki, A. et al. (2003) Neurosci. Lett. 338:17.
Long Name
Glucose Transporter Type 5
Entrez Gene IDs
6518 (Human); 56485 (Mouse)
Alternate Names
Fructose transporter; Glucose transporter type 5, small intestine; Glut5; GLUT-5; GLUT5glucose transporter-like protein 5; SLC2A5; solute carrier family 2 (facilitated glucose transporter), member 5; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; solute carrier family 2, facilitated glucose transporter member 5

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