Human Glut5 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Glut5. Stains human Glut5 transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Monoclonal Mouse IgG2A Clone # 195205
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Glut5
    Met1-Gln501
    Accession # P22732
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human small intestine and skeletal muscle
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Glut5 in NS0 Mouse Cell Line Transfected with Human Glut5 by Flow Cytometry. NS0 mouse myeloma cell line either transfected with human Glut5 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human Glut5 Monoclonal Antibody, followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glut5
Glut5 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues. Glut5 is expressed at highest levels in small intestine and at lower levels in kidney, testis, skeletal muscle, and adipose tissue (1, 2). Glut5 transports fructose in intestine, testis, and other tissues.
  • References:
    1. Kayano, T. et al. (1990) J. Biol. Chem. 265:13276.
    2. Corpe, C.P. (2002) Biochem. Biophys. Acta. 1576:191.
    3. Sasaki, A. et al. (2003) Neurosci. Lett. 338:17.
  • Long Name:
    Glucose Transporter Type 5
  • Entrez Gene IDs:
    6518 (Human); 56485 (Mouse)
  • Alternate Names:
    Fructose transporter; Glucose transporter type 5, small intestine; Glut5; GLUT-5; GLUT5glucose transporter-like protein 5; SLC2A5; solute carrier family 2 (facilitated glucose transporter), member 5; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; solute carrier family 2, facilitated glucose transporter member 5
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