Human Glut5 Biotinylated Antibody

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BAM1349
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Human Glut5 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glut5. Stains human Glut5-transfected NS0 cells; also detects Glut5 on human microglia cells (Sasaki, A. et al. (2003) Neurosci. Lett. 338(1):17).
Source
Monoclonal Mouse IgG2A Clone # 195205
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Glut5
Met1-Gln501
Accession # P22732
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human Glut5 transfected cells
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human small intestine and skeletal muscle

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glut5

Glut5 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains, and isexpressed at variable levels in many tissues. Glut5 is expressed at highest levels insmall intestine and at lower levels in kidney, testis, skeletal muscle and adipose tissue (1, 2). Glut5 transports fructose in intestine, testis and other tissues.

Long Name
Glucose Transporter Type 5
Entrez Gene IDs
6518 (Human); 56485 (Mouse)
Alternate Names
Fructose transporter; Glucose transporter type 5, small intestine; Glut5; GLUT-5; GLUT5glucose transporter-like protein 5; SLC2A5; solute carrier family 2 (facilitated glucose transporter), member 5; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; solute carrier family 2, facilitated glucose transporter member 5

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