Human Glycophorin A Alexa Fluor® 647-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Glycophorin A, the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells [Greaves, M.F. et al. (1983) Blood 61(4):645].
  • Source
    Monoclonal Mouse IgG1 Clone # R10
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Abelson mouse leukemia virus-induced human pre-B tumor cells
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Glycophorin A in TF‑1 Human Cell Line by Flow Cytometry. TF‑1 human erythroleukemic cell line was stained with Alexa Fluor® 647‑conjugated Mouse Anti-Human Glycophorin A Monoclonal Antibody (Catalog # FAB1228R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram).View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Glycophorin A
Glycophorin A, also designated CD235a, is the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells (1).
  • References:
    1. Greaves, M.F. et al. (1983) Blood 61(4):645.
  • Entrez Gene IDs:
    2993 (Human)
  • Alternate Names:
    CD235a antigen; CD235a; glycophorin A (includes MN blood group); glycophorin A (MNS blood group); Glycophorin A; glycophorin Erik; glycophorin MiI; glycophorin MiIII; glycophorin MiV; glycophorin MiX; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GpMiIII; GPSAT; GYPA; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); Mi.V glycoprotein (24 AA); MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR
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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 647-conjugated Antibody

Ctrl IC002R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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