Human Glycophorin A Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1228R-100UG
Detection  of Glycophorin A in TF‑1 Human Cell Line by Flow  Cytometry.
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Human Glycophorin A Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glycophorin A, the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells [Greaves, M.F. et al. (1983) Blood 61(4):645].
Source
Monoclonal Mouse IgG1 Clone # R10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Abelson mouse leukemia virus-induced human pre-B tumor cells
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of Glycophorin A in TF‑1 Human Cell Line by Flow  Cytometry. View Larger

Detection of Glycophorin A in TF‑1 Human Cell Line by Flow Cytometry. TF‑1 human erythroleukemic cell line was stained with Alexa Fluor® 647‑conjugated Mouse Anti-Human Glycophorin A Monoclonal Antibody (Catalog # FAB1228R, filled histogram) or isotype control antibody (Catalog # IC002R, open histogram).View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glycophorin A

Glycophorin A, also designated CD235a, is the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells (1).

References
  1. Greaves, M.F. et al. (1983) Blood 61(4):645.
Entrez Gene IDs
2993 (Human)
Alternate Names
CD235a antigen; CD235a; glycophorin A (includes MN blood group); glycophorin A (MNS blood group); Glycophorin A; glycophorin Erik; glycophorin MiI; glycophorin MiIII; glycophorin MiV; glycophorin MiX; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GpMiIII; GPSAT; GYPA; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); Mi.V glycoprotein (24 AA); MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR

Product Datasheets

FAQs

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