Home / Glycophorin A / Human Glycophorin A Antibody

Human Glycophorin A Antibody

Catalog #: MAB1228
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1228-SP
MAB1228-500
MAB1228-100
Detection of Glycophorin A in TF‑1 Human Cell Line by Flow Cytometry.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Glycophorin A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glycophorin A, the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells [Greaves, M.F. et al. (1983) Blood 61(4):645].
Source
Monoclonal Mouse IgG1 Clone # R10
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Abelson mouse leukemia virus-induced human pre-B tumor cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
Rearden, A. et al. (1985) Mol. Immunol. 22:369; San Miguel, J.F. et al. (1985) Br. J. Haematol. 59:297.
 
Immunoprecipitation
Blanchard, D. et al. (1983) Biochem. J. 213:399.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Glycophorin A antibody in TF-1 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Glycophorin A in TF‑1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Mouse Anti-Human Glycophorin A Monoclonal Antibody (Catalog # MAB1228, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunohistochemistry Glycophorin A antibody in Human Lung by Immunohistochemistry (IHC-P). View Larger

Glycophorin A in Human Lung. Glycophorin A was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human Glycophorin A Monoclonal Antibody (Catalog # MAB1228) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes of red blood cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glycophorin A

Glycophorin A, also designated CD235a, is the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells (1).

References
  1. Greaves, M.F. et al. (1983) Blood 61(4):645.
Entrez Gene IDs
2993 (Human)
Alternate Names
CD235a antigen; CD235a; glycophorin A (includes MN blood group); glycophorin A (MNS blood group); Glycophorin A; glycophorin Erik; glycophorin MiI; glycophorin MiIII; glycophorin MiV; glycophorin MiX; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GpMiIII; GPSAT; GYPA; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); Mi.V glycoprotein (24 AA); MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR

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Citations for Human Glycophorin A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Pathology-oriented multiplexing enables integrative disease mapping
    Authors: Kuehl, M;Okabayashi, Y;Wong, MN;Gernhold, L;Gut, G;Kaiser, N;Schwerk, M;Gräfe, SK;Ma, FY;Tanevski, J;Schäfer, PSL;Mezher, S;Sarabia Del Castillo, J;Goldbeck-Strieder, T;Zolotareva, O;Hartung, M;Delgado Chaves, FM;Klinkert, L;Gnirck, AC;Spehr, M;Fleck, D;Joodaki, M;Parra, V;Shaigan, M;Diebold, M;Prinz, M;Kranz, J;Kux, JM;Braun, F;Kretz, O;Wu, H;Grahammer, F;Heins, S;Zimmermann, M;Haas, F;Kylies, D;Wanner, N;Czogalla, J;Dumoulin, B;Zolotarev, N;Lindenmeyer, M;Karlson, P;Nyengaard, JR;Sebode, M;Weidemann, S;Wiech, T;Groene, HJ;Tomas, NM;Meyer-Schwesinger, C;Kuppe, C;Kramann, R;Karras, A;Bruneval, P;Tharaux, PL;Pastene, D;Yard, B;Schaub, JA;McCown, PJ;Pyle, L;Choi, YJ;Yokoo, T;Baumbach, J;Sáez, PJ;Costa, I;Turner, JE;Hodgin, JB;Saez-Rodriguez, J;Huber, TB;Bjornstad, P;Kretzler, M;Lenoir, O;Nikolic-Paterson, DJ;Pelkmans, L;Bonn, S;Puelles, VG;
    Nature
    Species: Human
    Sample Types: Whole Tissue
    Applications: IF/IHC
  2. The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis
    Authors: Oki? ?or?evi?, I;Kukolj, T;Živanovi?, M;Mom?ilovi?, S;Obradovi?, H;Petrovi?, A;Mojsilovi?, S;Trivanovi?, D;Jaukovi?, A;
    Biomolecules
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Application of polychromatic flow cytometry to identify novel subsets of circulating cells with angiogenic potential.
    Authors: Estes ML, Mund JA, Mead LE
    Cytometry A, 2010-09-01;77(9):831-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Endothelial abnormalities in adolescents with type 1 diabetes: a biomarker for vascular sequelae?
    Authors: DiMeglio LA, Tosh A, Saha C
    J. Pediatr., 2010-06-12;157(4):540-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. SnRNA sequencing defines signaling by RBC-derived extracellular vesicles in the murine heart
    Authors: Nedyalka Valkov, Avash Das, Nathan R Tucker, Guoping Li, Ane M Salvador, Mark D Chaffin et al.
    Life Science Alliance
  6. A human cell atlas of fetal gene expression
    Authors: Cao J, O'Day DR, Pliner HA et al.
    Science

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