Human Glycophorin A Alexa Fluor® 488-conjugated Antibody

FAB1228G Datasheet / COA / SDS

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FAB1228G-100UG

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Detection of Glycophorin A in TF-1 Human Cell Line by Flow Cytometry.

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Human Glycophorin A Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Glycophorin A, the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells [Greaves, M.F. et al. (1983) Blood 61(4):645].

Source

Monoclonal Mouse IgG₁ Clone # R10

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Abelson mouse leukemia virus-induced human pre-B tumor cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of Glycophorin A in TF-1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Alexa Fluor® 488-conjugated Mouse Anti-Human Glycophorin A Monoclonal Antibody (Catalog # FAB1228G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glycophorin A

Glycophorin A, also designated CD235a, is the major sialoglycoprotein expressed on red blood cells and erythroid precursor cells (1).

References

Greaves, M.F. et al. (1983) Blood 61(4):645.

Entrez Gene IDs

2993 (Human)

Alternate Names

CD235a antigen; CD235a; glycophorin A (includes MN blood group); glycophorin A (MNS blood group); Glycophorin A; glycophorin Erik; glycophorin MiI; glycophorin MiIII; glycophorin MiV; glycophorin MiX; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GpMiIII; GPSAT; GYPA; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); Mi.V glycoprotein (24 AA); MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR