Human Glycoprotein V/CD42d APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Glycoprotein V/CD42d in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human GPVI is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 508209
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Glycoprotein V/CD42d
    Accession # P40197
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Glycoprotein V/CD42d in Human Blood Platelets by Flow Cytometry. Human peripheral blood platelets were stained with anti-human CD41 FITC‑conjugated antibody and either (A) Mouse Anti-Human Glycoprotein V/CD42d APC‑conjugated Monoclonal Antibody (Catalog # FAB4249A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Glycoprotein V/CD42d

GPV (platelet glycoprotein V; designated CD42d) is an 83 kDa type I transmembrane (TM) glycoprotein of the leucine‑rich repeat (LRR) family (1, 2). It is expressed exclusively within the platelet / megakaryocyte lineage, where it noncovalently interacts with other platelet TM LRR proteins, GPIb alpha / beta and GPIX, at a ratio of one GPV to two of each other subunit (2). The GPI‑V‑IX complex tethers platelets to von Willebrand factor on the surface of injured endothelial cells. Absence of the complex results in Bernard‑Soulier syndrome, a rare bleeding disorder (1‑3). The human GPV cDNA encodes a 560 amino acid (aa) protein with a 16 aa signal sequence, a 507 aa extracellular domain (ECD) containing 15 LRR, a 21 aa TM sequence, and a short (16 aa) cytoplasmic tail that binds calmodulin in resting, but not activated platelets. The human GPV ECD shares 70%, 71% and 81% aa identity with mouse, rat and equine GPV, respectively. GPV can form soluble fragments of 80 kDa by ADAM10 or ADAM17 cleavage after P507, or 69 kDa by thrombin cleavage after R476 (1, 4, 5). High circulating soluble GPV may be an indicator of platelet activation, but may also be caused by high doses of aspirin (6‑8). The function of GPV is not entirely clear. Deletion of GPV in mice does not produce any obvious change to surface expression or function of GPIb and GPIX, but surface expression of GPV requires GPIb (9, 10). Deletion studies also indicate that GPV may play a minor role in collagen adhesion, and may modify platelet aggregation in response to thrombin (3, 11‑15).

  • References:
    1. Lanza, F. et al. (1993) J. Biol. Chem. 268:20801.
    2. Hickey, M.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:8327.
    3. Ozaki, Y. et al. (2005) J. Thromb. Haemost. 3:1745.
    4. Rabie, T. et al. (2005) J. Biol. Chem. 280:14462.
    5. Gardiner, E.E. et al. (2007) J. Thromb. Haemost. 5:1530.
    6. Wolff, V. et al. (2005) Stroke 36:e17.
    7. Javela, K. et al. (2005) Transfusion 45:1504.
    8. Aktas, B. et al. (2005) J. Biol. Chem. 280:39716.
    9. Kahn, M.L. (1999) Blood 94:4112.
    10. Strassel, C. et al. (2004) Eur. J. Biochem. 271:3671.
    11. Nonne, C. et al. (2008) J. Thromb. Haemost. 6:210.
    12. Moog, S. et al. (2001) Blood 98:1038.
    13. Ramakrishnan, V. et al. (1999) Proc. Natl. Acad. Sci. USA 96:13336.
    14. Ni, H. et al. (2001) Blood 98:368.
    15. Andrews, R.K. et al. (2001) Blood 98:681.
  • Entrez Gene IDs:
    2814 (Human); 14729 (Mouse); 25259 (Rat)
  • Alternate Names:
    platelet glycoprotein V; CD42d; glycoprotein V (platelet); GP5; GPV
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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