Human GPR12 Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6738V-100UG
R&D Systems Antibodies
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Human GPR12 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPR12 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 584940
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPR12
Met1-Val334
Accession # P47775
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
HEK293 Human Cell Line Transfected with human GRP12 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GPR12

GPR12 is a 7-transmembrane receptor for sphingosylphosphorylcholine. Human GPR12 and the related GPR3 and GPR6 are all highly expressed on neurons of the central nervous system, while GPR12 is particularly expressed in the limbic system. GPR12 engagement promotes proliferation and maturation of postmitotic neurons through an inhibitory G protein and cAMP signaling pathway. Human GPR12 cDNA encodes 334 amino acids (aa), 89 of which are extracellular; these share 86% aa identity with mouse and rat GPR12.

Long Name
G Protein-coupled Receptor 12
Entrez Gene IDs
2835 (Human); 14738 (Mouse); 80840 (Rat)
Alternate Names
FLJ18149; FLJ97704; G protein-coupled receptor 12; GPCR12; GPCR21; GPR12; G-protein coupled receptor 12; MGC138349; MGC138351

Product Datasheets

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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