Detection of GPR12 in HEK293 Human Cell Line Transfected with human GRP12 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) Human GRP12 or (B) irrelevant transfectant, and eGFP was stained with Mouse Anti-Human GPR12 Monoclonal Antibody (Catalog # MAB6738) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrants were set based on Mouse IgG1 Flow Cytometry Isotype Control (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.
GPR12 in Human Brain.
GPR12 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human GPR12 Monoclonal Antibody (Catalog # MAB6738) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
GPR12 is a 7-transmembrane receptor for sphingosylphosphorylcholine. Human GPR12 and the related GPR3 and GPR6 are all highly expressed on neurons of the central nervous system, while GPR12 is particularly expressed in the limbic system. GPR12 engagement promotes proliferation and maturation of postmitotic neurons through an inhibitory G protein and cAMP signaling pathway. Human GPR12 cDNA encodes 334 amino acids (aa), 89 of which are extracellular; these share 86% aa identity with mouse and rat GPR12.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.