Human GPR12 Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human GPR12 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 584940
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human GPR12
    Met1-Val334
    Accession # P47775
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of GPR12 in HEK293 Human Cell Line Transfected with human GRP12 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) Human GRP12 or (B) irrelevant transfectant, and eGFP was stained with Mouse Anti-Human GPR12 Monoclonal Antibody (Catalog # MAB6738) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrants were set based on Mouse IgG1 Flow Cytometry Isotype Control (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
GPR12 in Human Brain. GPR12 was detected in immersion fixed paraffin-embedded sections of human brain using Mouse Anti-Human GPR12 Monoclonal Antibody (Catalog # MAB6738) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR12
GPR12 is a 7-transmembrane receptor for sphingosylphosphorylcholine. Human GPR12 and the related GPR3 and GPR6 are all highly expressed on neurons of the central nervous system, while GPR12 is particularly expressed in the limbic system. GPR12 engagement promotes proliferation and maturation of postmitotic neurons through an inhibitory G protein and cAMP signaling pathway. Human GPR12 cDNA encodes 334 amino acids (aa), 89 of which are extracellular; these share 86% aa identity with mouse and rat GPR12.
  • Long Name:
    G Protein-coupled Receptor 12
  • Entrez Gene IDs:
    2835 (Human); 14738 (Mouse); 80840 (Rat)
  • Alternate Names:
    FLJ18149; FLJ97704; G protein-coupled receptor 12; GPCR12; GPCR21; GPR12; G-protein coupled receptor 12; MGC138349; MGC138351
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

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Mouse IgG1 Isotype Control

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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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