Human GPR12 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6738P-100
Detection of GPR12 in HEK293 Human Cell Line Transfected with Human GPR12 and eGFP by Flow Cytometry.
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Human GPR12 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPR12 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 584940
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPR12
Accession # P47775
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of GPR12 in HEK293 Human Cell Line Transfected with Human GPR12 and eGFP by Flow Cytometry. View Larger

Detection of GPR12 in HEK293 Human Cell Line Transfected with Human GPR12 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human GPR12 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human GPR12 PE-conjugated Monoclonal Antibody (Catalog # FAB6738P). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: GPR12

GPR12 is a 7-transmembrane receptor for sphingosylphosphorylcholine. Human GPR12 and the related GPR3 and GPR6 are all highly expressed on neurons of the central nervous system, while GPR12 is particularly expressed in the limbic system. GPR12 engagement promotes proliferation and maturation of postmitotic neurons through an inhibitory G protein and cAMP signaling pathway. Human GPR12 cDNA encodes 334 amino acids (aa), 89 of which are extracellular; these share 86% aa identity with mouse and rat GPR12.

Long Name
G Protein-coupled Receptor 12
Entrez Gene IDs
2835 (Human); 14738 (Mouse); 80840 (Rat)
Alternate Names
FLJ18149; FLJ97704; G protein-coupled receptor 12; GPCR12; GPCR21; GPR12; G-protein coupled receptor 12; MGC138349; MGC138351

Product Datasheets

FAQs

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