Human HAI-2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1106
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Human HAI-2 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human HAI-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse HAI-2B and recombinant human HAI-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAI-2A (R&D Systems, Catalog # 1106-PI)
Ala28-Lys197
Accession # O43291
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human HAI‑2

Human HAI-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human HAI‑2 Ectodomain Antibody (Catalog # MAB1106)

Standard: Recombinant Human HAI-2A Protein, CF (Catalog # 1106-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-2

Two alternatively spliced forms of HAI-2 have been found in human tissues (1). HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. Encoded by the SPINT2 gene, HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain (1 - 5). Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trysin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa. In comparison, HAI-2A is a weaker inhibitor of factor VIIa-tissue factor, factors IXa, Xa, and XIIa.

References
  1. Kataoka, H. et al. (2002) Biochem. Biophys. Res. Comm. 290:1096.
  2. Kawaguchi, T. et al. (1997) J. Biol. Chem. 272:27558.
  3. Marlor. C.W. et al. (1997) J. Biol. Chem. 272:12202.
  4. Muller-Pillasch, F. et al. (1998) Biochim. Biophys. Acta 1395:88.
  5. Delaria, K.A. et al. (1997) J. Biol. Chem. 272:12209.
Long Name
HGF Activator Inhibitor Type 2
Entrez Gene IDs
10653 (Human); 20733 (Mouse)
Alternate Names
HAI2; HAI-2; HAI-2DIAR3; HAI2FLJ45571; Hepatocyte growth factor activator inhibitor type 2; Kop; kunitz-type protease inhibitor 2; PB; Placental bikunin; serine peptidase inhibitor, Kunitz type, 2; serine protease inhibitor, Kunitz type, 2

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