Human HB-EGF Quantikine ELISA Kit DHBEG0: R&D Systems

Human HB-EGF Quantikine ELISA Kit

  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (100 uL), EDTA Plasma (100 uL), Platelet-poor EDTA Plasma (100 uL), Heparin Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL), Saliva (50 uL), Urine (100 uL), Human Milk (100 uL)
  • Sensitivity
    1.74 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva, Urine, Human Milk)
  • Specificity
    Natural and recombinant human HB-EGF.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC215, Quantikine Immunoassay Control Set 886 Human HB-EGF - Please Inquire
Product Summary
The Quantikine Human HB-EGF Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human HB-EGF in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains Sf 21-expressed recombinant human HB-EGF and antibodies raised against the recombinant factor. Results obtained using natural human HB-EGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring
human HB-EGF.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva, Urine, Human Milk
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean8218632876171313
Standard Deviation2.822.32.57.3110.122.2
CV%3.41.20.89.65.97.1

Recovery

The recovery of human HB-EGF spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 120 113-125
EDTA Plasma (n=4) 99 83-114
Heparin Plasma (n=4) 84 75-96
Platelet-poor EDTA Plasma (n=4) 101 84-119
Platelet-poor Heparin Plasma (n=4) 98 75-111
Serum (n=4) 95 79-107
Urine (n=4) 98 81-121
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human HB-EGF were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human HB-EGF Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HB-EGF
HB-EGF (Heparin-Binding EGF-like growth factor), also known as DTR, is produced by bronchial epithelium, visceral and vascular smooth muscle, CD4+ T cells, cardiac muscle, glomerular podocytes, keratinocytes, and IL-10-secreting regulatory macrophages. It activates both the EGF R/ErbB1 and ErbB4 receptors and has been linked to many cellular processes including proliferation, apoptosis, cell migration/invasion, differentiation, morphogenesis, and development. HB-EGF is also involved in several aspects of cancer development and progression.
    • Long Name
      Heparin Binding EGF-like Growth Factor
    • Entrez Gene IDs
      1839 (Human); 15200 (Mouse);
    • Alternate Names
      diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor); Dtr; DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor); Dts; DTSF; HBEGF; HB-EGF; Hegfl; heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; proheparin-binding EGF-like growth factor;
    Related Research Areas
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 886 Human HB-EGF

    Ctrl QC215
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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