HB-EGF (Heparin-Binding EGF-like growth factor), also known as DTR, is produced by bronchial epithelium, visceral and vascular smooth muscle, CD4+ T cells, cardiac muscle, glomerular podocytes, keratinocytes, and IL-10-secreting regulatory macrophages. It activates both the EGF R/ErbB1 and ErbB4 receptors and has been linked to many cellular processes including proliferation, apoptosis, cell migration/invasion, differentiation, morphogenesis, and development. HB-EGF is also involved in several aspects of cancer development and progression.
Human HB-EGF Quantikine ELISA Kit
R&D Systems | Catalog # DHBEG0
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (100 µL), EDTA Plasma (100 µL), Platelet-poor EDTA Plasma (100 µL), Heparin Plasma (100 µL), Platelet-poor Heparin Plasma (100 µL), Saliva (50 µL), Urine (100 µL), Human Milk (100 µL)
Sensitivity
1.74 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva, Urine, Human Milk)
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Product Summary for Human HB-EGF Quantikine ELISA Kit
The Quantikine Human HB-EGF Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human HB-EGF in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains Sf 21-expressed recombinant human HB-EGF and antibodies raised against the recombinant factor. Results obtained using natural human HB-EGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring
human HB-EGF.
human HB-EGF.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human HB-EGF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Human Milk, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 82.0 | 186 | 328 | 76.0 | 171 | 313 |
| Standard Deviation | 2.82 | 2.30 | 2.50 | 7.31 | 10.1 | 22.2 |
| CV% | 3.4 | 1.2 | 0.8 | 9.6 | 5.9 | 7.1 |
Recovery for Human HB-EGF Quantikine ELISA Kit
The recovery of human HB-EGF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 120 | 113-125 |
| EDTA Plasma (n=4) | 99 | 83-114 |
| Heparin Plasma (n=4) | 84 | 75-96 |
| Platelet-poor EDTA Plasma (n=4) | 101 | 84-119 |
| Platelet-poor Heparin Plasma (n=4) | 98 | 75-111 |
| Serum (n=4) | 95 | 79-107 |
| Urine (n=4) | 98 | 81-121 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human HB-EGF were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human HB-EGF Quantikine ELISA Kit
Human HB-EGF ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HB-EGF
Long Name
Heparin Binding EGF-like Growth Factor
Alternate Names
Dtr, Dts, HBEGF, Hegfl
Gene Symbol
HBEGF
Additional HB-EGF Products
Product Documents for Human HB-EGF Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human HB-EGF Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Human HB-EGF Quantikine ELISA Kit
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
