Human HB-EGF Quantikine ELISA Kit Summary
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (100 uL), EDTA Plasma (100 uL), Platelet-poor EDTA Plasma (100 uL), Heparin Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL), Saliva (50 uL), Urine (100 uL), Human Milk (100 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human HB-EGF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human HB-EGF Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human HB-EGF in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains Sf 21-expressed recombinant human HB-EGF and antibodies raised against the recombinant factor. Results obtained using natural human HB-EGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring
human HB-EGF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Platelet-poor EDTA Plasma, Heparin Plasma, Platelet-poor Heparin Plasma, Saliva, Urine, Human Milk
|
Intra-Assay Precision |
Inter-Assay Precision |
| Sample |
1 |
2 |
3 |
1 |
2 |
3 |
| n |
20 |
20 |
20 |
20 |
20 |
20 |
| Mean |
82 |
186 |
328 |
76 |
171 |
313 |
| Standard Deviation |
2.82 |
2.3 |
2.5 |
7.31 |
10.1 |
22.2 |
| CV% |
3.4 |
1.2 |
0.8 |
9.6 |
5.9 |
7.1 |
Recovery
The recovery of human HB-EGF spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type |
Average % Recovery |
Range % |
| Cell Culture Media (n=4) |
120 |
113-125 |
| EDTA Plasma (n=4) |
99 |
83-114 |
| Heparin Plasma (n=4) |
84 |
75-96 |
| Platelet-poor EDTA Plasma (n=4) |
101 |
84-119 |
| Platelet-poor Heparin Plasma (n=4) |
98 |
75-111 |
| Serum (n=4) |
95 |
79-107 |
| Urine (n=4) |
98 |
81-121 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human HB-EGF were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Data Examples
Human HB-EGF ELISA Standard Curve
Preparation and Storage
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HB-EGF
HB-EGF (Heparin-Binding EGF-like growth factor), also known as DTR, is produced by bronchial epithelium, visceral and vascular smooth muscle, CD4+ T cells, cardiac muscle, glomerular podocytes, keratinocytes, and IL-10-secreting regulatory macrophages. It activates both the EGF R/ErbB1 and ErbB4 receptors and has been linked to many cellular processes including proliferation, apoptosis, cell migration/invasion, differentiation, morphogenesis, and development. HB-EGF is also involved in several aspects of cancer development and progression.
Long Name:
Heparin Binding EGF-like Growth Factor
Entrez Gene IDs:
1839 (Human); 15200 (Mouse)
Alternate Names:
diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor); Dtr; DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor); Dts; DTSF; HBEGF; HB-EGF; Hegfl; heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; proheparin-binding EGF-like growth factor
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
