Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all HES-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HES-4
Val94-Arg221
Accession # Q9HCC6

Specificity

Detects human HES-4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) HES-1, rhHES-2, rhHES-5, and rhHES-7 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human HES-4 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human HES‑4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HES-4

HES-4 is a 221 aa, 24 kDa member of the HES-family of transcriptional repressors. HES-4 contains a species-specific bHLH domain (aa 33‑93), a specific orange DNA-binding domain (aa 109‑150), a 65 aa Proline-rich SH3-binding domain, and a WRPW Groucho/TLE interacting C-terminal motif. HES-4 is a HES-1 paralog likely to maintain stem cell pluripotency. In the region used as immunogen, the amino acid sequence of human and canine HES-4 are 80% identical.

Long Name

Hairy And Enhancer of Split 4

Alternate Names

HES4

Entrez Gene IDs

57801 (Human)

Gene Symbol

HES4

UniProt

Q9HCC6

Additional HES-4 Products

Product Documents for Human HES-4 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HES-4 Biotinylated Antibody

For research use only

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FAQs

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