Human HES-4 Biotinylated Antibody

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Human HES-4 Biotinylated Antibody Summary

Species Reactivity
Detects human HES-4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) HES-1, rhHES-2, rhHES-5, and rhHES-7 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human HES-4
Accession # Q9HCC6
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human HES‑4

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HES-4

HES-4 is a 221 aa, 24 kDa member of the HES-family of transcriptional repressors. HES-4 contains a species-specific bHLH domain (aa 33‑93), a specific orange DNA-binding domain (aa 109‑150), a 65 aa Proline-rich SH3-binding domain, and a WRPW Groucho/TLE interacting C-terminal motif. HES-4 is a HES-1 paralog likely to maintain stem cell pluripotency. In the region used as immunogen, the amino acid sequence of human and canine HES-4 are 80% identical.

Long Name
Hairy And Enhancer of Split 4
Entrez Gene IDs
57801 (Human)
Alternate Names
bHLH factor Hes4; BHLHB42; bHLHb42transcription factor HES-4; Class B basic helix-loop-helix protein 42; hairy and enhancer of split 4 (Drosophila); Hairy and enhancer of split 4; HES4; HES-4; hHES4

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