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Human HGF Quantikine ELISA Kit

R&D Systems | Catalog # DHG00B

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Key Product Details

Assay Length

4.0 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Citrate Plasma (50 µL)

Sensitivity

24.1 pg/mL

Assay Range

156-10000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
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Product Summary for Human HGF Quantikine ELISA Kit

The Quantikine® Human HGF Immunoassay is a 4.0 hour solid phase ELISA that is designed to measure human HGF levels in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human HGF and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human HGF. Results obtained using natural human HGF showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human HGF.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human HGF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 1296 2626 5080 1414 2755 5410
Standard Deviation 30.2 75.8 147 112 176 212
CV% 2.3 2.9 2.9 7.9 6.4 3.9

Recovery for Human HGF Quantikine ELISA Kit

The recovery of human HGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 107 101-118
Citrate Plasma (n=4) 102 91-115
EDTA Plasma (n=4) 104 95-114
Heparin Plasma (n=4) 113 96-125
Serum (n=4) 104 94-115

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human HGF were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human HGF Linearity
Human HGF ELISA Linearity

Scientific Data Images for Human HGF Quantikine ELISA Kit

Human HGF ELISA Cell Culture Supernate Standard Curve

Human HGF ELISA Cell Culture Supernate Standard Curve

Human HGF ELISA Standard Curve

Human HGF ELISA Standard Curve

Human HGF ELISA Serum/Plasma Standard Curve

Human HGF ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HGF

HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.

Long Name

Hepatocyte Growth Factor

Alternate Names

DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF

Entrez Gene IDs

3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)

Gene Symbol

HGF

Additional HGF Products

Product Documents for Human HGF Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HGF Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human HGF Quantikine ELISA Kit

Customer Reviews for Human HGF Quantikine ELISA Kit (2)

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  • Human HGF Quantikine ELISA Kit
    Name: Vincent Giacalone
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 05/15/2023
    This ELISA kit is straightforward and reliable. Sometimes there are precipitates in the wash buffer that will not fully dissolve unless the bottle is first incubated at 37C in bead/water bath. works great for cell culture supernatants
    Human HGF Quantikine ELISA Kit DHG00B
  • Human HGF Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 02/25/2023

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