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Human HNF-4 alpha /NR2A1 Antibody

Catalog #: PP-H1415-00
14 Citations Datasheet / COA / SDS
Newer Version Available: PP-H1415-0C
NEW
Isoforms 1, 2, 4, 5, 7, 8

Discontinued Product

PP-H1415-00 has been discontinued and is replaced by PP-H1415-0C.

Product Details
Citations (14)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HNF-4 alpha /NR2A1 Antibody Summary

Species Reactivity
Human
Specificity
This antibody specifically recognizes human HNF4 alpha (isoforms 1, 2, 4, 5, 7 and 8) and cross-reacts with mouse and rat HNF4 alpha (isoforms 1, 2, 4, 5, 7 and 8). Not yet tested in other species.
Source
Monoclonal Mouse IgG2A Clone # H1415
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human HNF-4 alpha/NR2A1
aa 393-461
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
This antibody can be used at 1 μg/mL under reducing conditions with the appropriate secondary reagents to detect human HNF4 alpha. Under non-reducing conditions, optimal dilutions should be determined by each laboratory.
 
Immunohistochemistry
This antibody can be used at 10 - 20 μg/mL withthe appropriate secondary reagents to detect human HNF4 alpha.
 
Immunoprecipitation
Optimal dilutions should be determined by eachlaboratory.
 
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions should be determined byeach laboratory.
 
Direct ELISA
This antibody can be used at 0.3 μg/mL with the appropriatesecondary reagents to detect human HNF4 alpha.
 
Gel Supershift Assay
This antibody can be used at 2 ng/assay to detect humanHNF4 alpha.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: HNF-4 alpha/NR2A1

Hepatocyte Nuclear Factor 4 alpha (HNF4, HNF4 alpha ; NR2A1) is a member of the Orphan Nuclear Receptor family. HNF4 is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9.

Long Name
Hepatocyte Nuclear Factor-4, alpha
Entrez Gene IDs
3172 (Human)
Alternate Names
FLJ39654; hepatic nuclear factor 4 alpha; hepatocyte nuclear factor 4, alpha; hepatocyte nuclear factor 4-alpha; HNF4 alpha; HNF-4 alpha; HNF4A; HNF4a7; HNF-4-alpha; HNF4alpha10/11/12; HNF4HNF4a8; MODY; MODY1; NR2A1; NR2A1HNF4a9; NR2A21; Nuclear receptor subfamily 2 group A member 1; TCF; TCF14; TCF-14; TCF14HNF4alpha; Transcription factor 14; Transcription factor HNF-4; transcription factor-14

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Citations for Human HNF-4 alpha /NR2A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. HCF-1 as a key modulator of OGT function and O-GlcNAcylation in the liver
    Authors: Kapuria, V;Ayushma, ;Herr, W;Minocha, S;
    Scientific reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Integration of Kupffer cells into human iPSC-derived liver organoids for modeling liver dysfunction in sepsis
    Authors: Li, Y;Nie, Y;Yang, X;Liu, Y;Deng, X;Hayashi, Y;Plummer, R;Li, Q;Luo, N;Kasai, T;Okumura, T;Kamishibahara, Y;Komoto, T;Ohkuma, T;Okamoto, S;Isobe, Y;Yamaguchi, K;Furukawa, Y;Taniguchi, H;
    Cell reports
    Species: Human
    Sample Types: Organoid
    Applications: Immunohistochemistry
  3. Regulatory chromatin rewiring promotes metabolic switching during adaptation to oncogenic receptor tyrosine kinase inhibition
    Authors: S Ogden, K Carys, I Ahmed, J Bruce, AD Sharrocks
    Oncogene, 2022-09-24;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP, Western Blot
  4. A potent HNF4alpha agonist reveals that HNF4alpha controls genes important in inflammatory bowel disease and Paneth cells
    Authors: SH Lee, V Veeriah, F Levine
    PLoS ONE, 2022-04-06;17(4):e0266066.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Liver fat storage is controlled by HNF4&alpha through induction of lipophagy and is reversed by a potent HNF4&alpha agonist
    Authors: SH Lee, V Veeriah, F Levine
    Cell Death & Disease, 2021-06-11;12(6):603.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Adeno-Associated Virus Serotype 8-Mediated Genetic Labeling of Cholangiocytes in the Neonatal Murine Liver
    Authors: Sanghoon Lee, Ping Zhou, Senyo Whyte, Soona Shin
    Pharmaceutics
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  7. Associating liver partition and portal vein ligation for staged hepatectomy: establishment of an animal model with insufficient liver remnant
    Authors: A Dili, V Lebrun, C Bertrand, IA Leclercq
    Lab. Invest., 2019-01-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  8. Bile acids regulate cysteine catabolism and glutathione regeneration to modulate hepatic sensitivity to oxidative injury
    Authors: Y Wang, J Li, D Matye, Y Zhang, K Dennis, WX Ding, T Li
    JCI Insight, 2018-04-19;3(8):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ChIP
  9. Genomic variants link to hepatitis C racial disparities
    Authors: MM Yeh, S Boukhar, B Roberts, N Dasgupta, SS Daoud
    Oncotarget, 2017-08-01;8(35):59455-59475.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. Epiblast-specific loss of HCF-1 leads to failure in anterior-posterior axis specification
    Dev Biol, 2016-08-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Opposing roles of nuclear receptor HNF4 alpha isoforms in colitis and colitis-associated colon cancer
    Authors: Karthikeyani Chellappa, Poonamjot Deol, Jane R Evans, Linh M Vuong, Gang Chen, Nadege Briançon et al.
    eLife
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: Immunohistochemistry, Western Blot
  12. Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment.
    Authors: Mu X, Espanol-Suner R, Mederacke I, Affo S, Manco R, Sempoux C, Lemaigre F, Adili A, Yuan D, Weber A, Unger K, Heikenwalder M, Leclercq I, Schwabe R
    J Clin Invest, 2015-09-08;125(10):3891-903.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Human but not mouse hepatocytes respond to interferon-lambda in vivo.
    Authors: Hermant P, Demarez C, Mahlakoiv T, Staeheli P, Meuleman P, Michiels T
    PLoS ONE, 2014-01-31;9(1):e87906.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Identification of a binding motif specific to HNF4 by comparative analysis of multiple nuclear receptors
    Authors: Bin Fang, Daniel Mane-Padros, Eugene Bolotin, Tao Jiang, Frances M. Sladek
    Nucleic Acids Research
    Species: Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Types: Nuclear Extract
    Applications: Bioassay

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