Human HNF‑4 alpha /NR2A1 Antibody

R&D Systems | Catalog # PP-H1415-00

Isoforms 1, 2, 4, 5, 7, 8
R&D Systems
Discontinued Product
PP-H1415-00 has been discontinued. An alternative/replacement product is available: PP-H1415-0C. View all HNF-4 alpha/NR2A1 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Chlorocebus aethiops (African Green Monkey), Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Direct ELISA, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Gel Supershift Assay

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP), Bioassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # H1415
View all HNF-4 alpha/NR2A1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human HNF-4 alpha/NR2A1
aa 393-461

Specificity

This antibody specifically recognizes human HNF4 alpha (isoforms 1, 2, 4, 5, 7 and 8) and cross-reacts with mouse and rat HNF4 alpha (isoforms 1, 2, 4, 5, 7 and 8). Not yet tested in other species.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human HNF‑4 alpha /NR2A1 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions should be determined by each laboratory.

Direct ELISA

This antibody can be used at 0.3 μg/mL with the appropriate secondary reagents to detect human HNF4 alpha.

Gel Supershift Assay

This antibody can be used at 2 ng/assay to detect human HNF4 alpha.

Immunohistochemistry

This antibody can be used at 10 - 20 μg/mL with the appropriate secondary reagents to detect human HNF4 alpha.

Immunoprecipitation

Optimal dilutions should be determined by each laboratory.

Western Blot

This antibody can be used at 1 μg/mL under reducing conditions with the appropriate secondary reagents to detect human HNF4 alpha. Under non-reducing conditions, optimal dilutions should be determined by each laboratory.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: HNF-4 alpha/NR2A1

Hepatocyte Nuclear Factor 4 alpha (HNF4, HNF4 alpha ; NR2A1) is a member of the Orphan Nuclear Receptor family. HNF4 is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Alternate Names

HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14

Entrez Gene IDs

3172 (Human)

Gene Symbol

HNF4A

Additional HNF-4 alpha/NR2A1 Products

Product Documents for Human HNF‑4 alpha /NR2A1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HNF‑4 alpha /NR2A1 Antibody

For research use only

Citations for Human HNF‑4 alpha /NR2A1 Antibody

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail