Human HVEM/TNFRSF14 Alexa Fluor® 700-conjugated Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human HVEM/TNFRSF14 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CD30 and no cross-reactivity with rh4-1BB, rhCD27, rhCD40, rhDR3, rhDR6, rhFas, rhGITR, rhNGF-R, rhOPG, rhRANK, recombinant mouse (rm) RANK, rhTAJ, rhTNF RI, rhTNF RII, or rmTNF RI is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 94801
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human HVEM/TNFRSF14
    Pro37-Val202 (Ser108Thr and Ala140Arg)
    Accession # AAB58354
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    Human peripheral blood lymphocytes
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HVEM/TNFRSF14

Herpesvirus entry mediator (HVEM), also referred to as TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. In the TNF superfamily nomenclature, HVEM is referred to as TNFRSF14. Human HVEM cDNA encodes a 283 amino acid (aa) protein with a probable 36 aa signal peptide, a 166 aa extracellular domain, a 23 aa transmembrane region and a 58 aa cytoplasmic region. The extracellular domain of HVEM contains several cysteine-rich repeats characteristic of TNF receptor superfamily members. The short cytoplasmic region lacks a death domain present in some TNF receptor family members, but contains a TRAF (TNF receptor-associated factor) interaction domain. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D. Two TNF superfamily ligands, including the secreted TNF‑ beta  (lymphotoxin  alpha ) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LT beta R, the receptor for lymphotoxin alpha beta heterotrimer.

  • References:
    1. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
    2. Mauri, D.N. et al. (1998) Immunity 8:21.
    3. Montgomery, R.I. et al. (1996) Cell 87:427.
  • Long Name:
    Herpesvirus Entry Mediator
  • Entrez Gene IDs:
    8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)
  • Alternate Names:
    ATAR; CD270 antigen; CD270; CD40-like protein; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; HVEM; HVEMTR2HVEAATAR; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator); Tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2
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Isotype Controls
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Mouse IgG1 Alexa Fluor® 700-conjugated Antibody

Ctrl IC002N 1  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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