Human HVEM/TNFRSF14 APC-conjugated Antibody

(3 citations)   
  • Species Reactivity
  • Specificity
    Detects human HVEM/TNFRSF14 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CD30 and no cross-reactivity with rh4-1BB, rhCD27, rhCD40, rhDR3, rhDR6, rhFas, rhGITR, rhNGF-R, rhOPG, rhRANK, recombinant mouse (rm) RANK, rhTAJ, rhTNF RI, rhTNF RII, or rmTNF RI is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 94801
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human HVEM/TNFRSF14
    Pro37-Val202 (Ser108Thr and Ala140Arg)
    Accession # AAB58354
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of HVEM/TNFRSF14 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HVEM/TNFRSF14 APC‑conjugated Monoclonal Antibody (Catalog # FAB356A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: HVEM/TNFRSF14

Herpesvirus entry mediator (HVEM), also known as TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. In the TNF superfamily nomenclature, HVEM is referred to as TNFRSF14. Human HVEM cDNA encodes a 283 amino acid (aa) protein with a probable 36 aa signal peptide, a 166 aa extracellular domain, a 23 aa transmembrane region and a 58 aa cytoplasmic region. The extracellular domain of HVEM contains several cysteine-rich repeats characteristic of TNF receptor superfamily members. The short cytoplasmic region lacks a death domain present in some TNF receptor family members, but contains a TRAF (TNF receptor-associated factor) interaction domain. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D. Two TNF superfamily ligands, including the secreted TNF‑ beta  (also known as lymphotoxin  alpha ) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LT beta R, the receptor for lymphotoxin alpha beta heterotrimer.

  • References:
    1. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
    2. Mauri, D.N. et al. (1998) Immunity 8:21.
    3. Montgomery, R.I. et al. (1996) Cell 87:427.
  • Long Name:
    Herpesvirus Entry Mediator
  • Entrez Gene IDs:
    8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)
  • Alternate Names:
    ATAR; CD270 antigen; CD270; CD40-like protein; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; HVEMTR2HVEAATAR; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator); Tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1.
    BMC Cancer, 2012;12(0):176.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. 4-1BBL induces TNF receptor-associated factor 1-dependent Bim modulation in human T cells and is a critical component in the costimulation-dependent rescue of functionally impaired HIV-specific CD8 T cells.
    Authors: Wang C, Wen T, Routy JP, Bernard NF, Sekaly RP, Watts TH
    J. Immunol., 2007;179(12):8252-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Telomere length of transferred lymphocytes correlates with in vivo persistence and tumor regression in melanoma patients receiving cell transfer therapy.
    Authors: Zhou J, Shen X, Huang J, Hodes RJ, Rosenberg SA, Robbins PF
    J. Immunol., 2005;175(10):7046-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 5  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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