Human HVEM/TNFRSF14 Antibody

Catalog # Availability Size / Price Qty
MAB356-SP
MAB356-500
MAB356-100
Detection  of HVEM/TNFRSF14 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (4)
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Human HVEM/TNFRSF14 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HVEM/TNFRSF14 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CD30 and no cross-reactivity with rh4-1BB, rhCD27, rhCD40, rhDR3, rhDR6, rhFas, rhGITR, rhNGF-R, rhOPG, rhRANK, recombinant mouse (rm) RANK, rhTAJ, rhTNF RI, rhTNF RII, or rmTNF RI is observed.
Source
Monoclonal Mouse IgG1 Clone # 94801
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HVEM/TNFRSF14
Pro37-Val202 (Ser108Thr and Ala140Arg)
Accession # AAB58354
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human HVEM/TNFRSF14 Fc Chimera (Catalog # 356-HV)
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human HVEM/TNFRSF14 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human HVEM/TNFRSF14 Biotinylated Antibody (Catalog # BAF356)

Standard: Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein (Catalog # 356-HV)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of HVEM/TNFRSF14 in Human Blood Lymphocytes by Flow Cytometry. View Larger

Detection of HVEM/TNFRSF14 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB356, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HVEM/TNFRSF14

Herpesvirus entry mediator (HVEM), also referred to as TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. In the TNF superfamily nomenclature, HVEM is referred to as TNFRSF14. Human HVEM cDNA encodes a 283 amino acid (aa) protein with a probable 36 aa signal peptide, a 166 aa extracellular domain, a 23 aa transmembrane region and a 58 aa cytoplasmic region. The extracellular domain of HVEM contains several cysteine-rich repeats characteristic of TNF receptor superfamily members. The short cytoplasmic region lacks a death domain present in some TNF receptor family members, but contains a TRAF (TNF receptor-associated factor) interaction domain. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D. Two TNF superfamily ligands, including the secreted TNF‑ beta  (lymphotoxin  alpha ) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LT beta R, the receptor for lymphotoxin alpha beta heterotrimer.

References
  1. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
  2. Mauri, D.N. et al. (1998) Immunity 8:21.
  3. Montgomery, R.I. et al. (1996) Cell 87:427.
Long Name
Herpesvirus Entry Mediator
Entrez Gene IDs
8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)
Alternate Names
ATAR; CD270 antigen; CD270; CD40-like protein; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; HVEM; HVEMTR2HVEAATAR; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator); Tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2

Product Datasheets

Citations for Human HVEM/TNFRSF14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Cytomegalovirus-Induced Expression of CD244 after Liver Transplantation Is Associated with CD8+ T Cell Hyporesponsiveness to Alloantigen.
    Authors: de Mare-Bredemeijer E, Shi X, Mancham S, van Gent R, van der Heide-Mulder M, de Boer R, Heemskerk M, de Jonge J, van der Laan L, Metselaar H, Kwekkeboom J
    J Immunol, 2015;195(4):1838-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  2. CD160 isoforms and regulation of CD4 and CD8 T-cell responses.
    Authors: El-Far, Mohamed, Pellerin, Charles, Pilote, Louise, Fortin, Jean-Fra, Lessard, Ivan A D, Peretz, Yoav, Wardrop, Elizabet, Salois, Patrick, Bethell, Richard, Cordingley, Michael, Kukolj, George
    J Transl Med, 2014;12(0):217.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  3. T cell intrinsic heterodimeric complexes between HVEM and BTLA determine receptivity to the surrounding microenvironment.
    Authors: Cheung TC, Oborne LM, Steinberg MW
    J. Immunol., 2009;183(11):7286-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  4. LIGHT up-regulated on B lymphocytes and monocytes in rheumatoid arthritis mediates cellular adhesion and metalloproteinase production by synoviocytes.
    Authors: Kang YM, Kim SY, Kang JH
    Arthritis Rheum., 2007;56(4):1106-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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Secondary Antibodies

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