Human ICAP-1 Antibody

Catalog # Availability Size / Price Qty
AF6805
AF6805-SP
Detection of Human ICAP‑1 by Western Blot.
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Product Details
Citations (1)
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Human ICAP-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAP-1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ICAP-1
Met1-Tyr127
Accession # O14713
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human ICAP‑1 by Western Blot. View Larger

Detection of Human ICAP‑1 by Western Blot. Western blot shows lysates of human small intestine tissue, human testis tissue, human spleen tissue, and human prostate tissue. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human ICAP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6805) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ICAP‑1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry ICAP-1 in Human Colon Cancer Tissue. View Larger

ICAP-1 in Human Colon Cancer Tissue. ICAP-1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Sheep Anti-Human ICAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6805) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAP-1

ICAP-1 (Integrin cytoplasmic domain-associated protein-1; also ITBP1) is a 22-27 kDa protein that specifically binds to the cytoplasmic tail of beta 1 integrin. It is widely expressed, and serves to negatively regulate cell migration/proliferation. In the cytosol, it has at least two functions. First, it associates with beta 1 integrin, promoting an inactive conformation. This has the effect of both slowing cell migration and allowing for the monitoring of ECM density. Second, it induces Delta-Notch signaling, an event that specifies the growth of only tip endothelium during angiogenesis. In the nucleus, ICAP-1 is proposed to participate in c-myc activation. Human ICAP-1 is 200 amino acids (aa) in length. It contains an NLS (aa 5-9) and one pleckstrin homology-like/PID domain (aa 58-200). Phosphorylation occurs at Thr38 and Ser41. There is one nonintegrin-binding alternative splice form that shows a deletion of aa 128-177. Over aa 1-127, human ICAP-1 shares 93% aa identity with mouse ICAP-1.

Long Name
Integrin Cytoplasmic Domain-associated Protein 1
Entrez Gene IDs
9270 (Human); 16413 (Mouse); 298914 (Rat)
Alternate Names
Bodenin; DKFZp686K08158; ICAP1; ICAP-1; ICAP-1alpha; ICAP-1B; ICAP1ICAP1AICAP1BICAP-1A; integrin beta 1 binding protein 1; integrin beta-1-binding protein 1; Integrin cytoplasmic domain-associated protein 1; integrin cytoplasmic domain-associated protein 1-alpha; integrin cytoplasmic domain-associated protein 1-beta; ITGB1BP1

Product Datasheets

Citation for Human ICAP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Nuclear Localization of Integrin Cytoplasmic Associated Protein-1 Influences ?1 Integrin Activation and Recruits Krev/ Interaction Trapped-1 to the Nucleus
    Authors: Kyle M Draheim
    J. Biol. Chem, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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