Human ICAP-1 Antibody

R&D Systems | Catalog # AF6805

R&D Systems
Discontinued Product
AF6805 has been discontinued. View all ICAP-1 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all ICAP-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ICAP-1
Met1-Tyr127
Accession # O14713

Specificity

Detects human ICAP-1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ICAP-1 Antibody

Detection of Human ICAP-1 antibody by Western Blot.

Detection of Human ICAP‑1 by Western Blot.

Western blot shows lysates of human small intestine tissue, human testis tissue, human spleen tissue, and human prostate tissue. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human ICAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6805) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ICAP-1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ICAP-1 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P).

ICAP-1 in Human Colon Cancer Tissue.

ICAP-1 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Sheep Anti-Human ICAP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6805) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human ICAP-1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue

Western Blot

2 µg/mL
Sample: Human small intestine tissue, human testis tissue, human spleen tissue, and human prostate tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICAP-1

ICAP-1 (Integrin cytoplasmic domain-associated protein-1; also ITBP1) is a 22-27 kDa protein that specifically binds to the cytoplasmic tail of beta 1 integrin. It is widely expressed, and serves to negatively regulate cell migration/proliferation. In the cytosol, it has at least two functions. First, it associates with beta 1 integrin, promoting an inactive conformation. This has the effect of both slowing cell migration and allowing for the monitoring of ECM density. Second, it induces Delta-Notch signaling, an event that specifies the growth of only tip endothelium during angiogenesis. In the nucleus, ICAP-1 is proposed to participate in c-myc activation. Human ICAP-1 is 200 amino acids (aa) in length. It contains an NLS (aa 5-9) and one pleckstrin homology-like/PID domain (aa 58-200). Phosphorylation occurs at Thr38 and Ser41. There is one nonintegrin-binding alternative splice form that shows a deletion of aa 128-177. Over aa 1-127, human ICAP-1 shares 93% aa identity with mouse ICAP-1.

Long Name

Integrin Cytoplasmic Domain-associated Protein 1

Alternate Names

Bodenin, ICAP1, ITGB1BP1

Entrez Gene IDs

9270 (Human); 16413 (Mouse); 298914 (Rat)

Gene Symbol

ITGB1BP1

UniProt

O14713 (Human)

Additional ICAP-1 Products

Product Documents for Human ICAP-1 Antibody

Certificate of Analysis

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Product Specific Notices for Human ICAP-1 Antibody

For research use only

Related Research Areas

Integrins and Associated Molecules

Citations for Human ICAP-1 Antibody

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Protocols

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