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Human IFN-beta Quantikine QuicKit ELISA

Catalog #: QK410
2 Citations Datasheet / COA / SDS
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QK410
Control Products Available
QC284: Human IFN-beta Quantikine QuicKit Control Group 284
Human IFN-beta Standard Curve ELISA
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Product Details
Citations (2)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IFN-beta Quantikine QuicKit ELISA Summary

Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL)
Sensitivity
2.88 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates)
Specificity
Natural and recombinant human IFN-beta.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine™ QuicKit™ Human IFN-beta Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IFN-beta levels in cell culture supernates. It contains CHO cell-expressed recombinant human IFN-beta and antibodies raised against the recombinant protein. Results obtained using natural human IFN-beta showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-beta.

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 20 20 10 10
Mean (pg/mL) 101 613 121 669
Standard Deviation 3.82 30.1 6.48 33.3
CV% 3.8 4.9 5.4 5

Recovery

The recovery of human IFN-beta spiked to three levels in samples throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Serum (n=)

Linearity

To assess the linearity of the assay, samples containing high concentrations of human IFN-beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IFN-beta Linearity ELISA

Scientific Data

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Human IFN-beta Standard Curve ELISA

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Long Name:
Interferon beta
Entrez Gene IDs:
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names:
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Citations for Human IFN-beta Quantikine QuicKit ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Mechanisms regulating transitory suppressive activity of neutrophils in newborns: PMNs-MDSCs in newborns
    Authors: M Perego, S Fu, Y Cao, A Kossenkov, M Yao, K Alicea-Tor, W Liu, Z Jiang, Z Chen, SY Fuchs, J Zhou, DI Gabrilovic
    Oncogene, 2022-06-21;0(0):.
    Species: Human
    Sample Types: Plasma
  2. Coronavirus induces diabetic macrophage-mediated inflammation via SETDB2
    Authors: WJ Melvin, CO Audu, FM Davis, SB Sharma, A Joshi, A DenDekker, S Wolf, E Barrett, K Mangum, X Zhou, M Bame, A Ruan, A Obi, SL Kunkel, BB Moore, KA Gallagher
    Proceedings of the National Academy of Sciences of the United States of America, 2021-09-21;118(38):.
    Species: Human
    Sample Types: Serum

FAQs

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ELISA Controls

Human IFN-beta Quantikine QuicKit Control Group 284

QC284
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