Human IFN-beta Quantikine QuicKit ELISA

Name: Human IFN-beta Quantikine QuicKit ELISA
Brand: R&D Systems
SKU: QK410
Price: 720 USD
Availability: InStock

R&D Systems | Catalog # QK410

Submit a review

Citations (13)

Product Documents

100% Guarantee

Product Summary
Product Specifications
Sample Values
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Assay Length

80 minutes

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL)

Sensitivity

2.88 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates)

View all IFN-beta ELISA Kits »

Product Summary for Human IFN-beta Quantikine QuicKit ELISA

The Quantikine™ QuicKit™ Human IFN-beta Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IFN-beta levels in cell culture supernates. It contains CHO cell-expressed recombinant human IFN-beta and antibodies raised against the recombinant protein. Results obtained using natural human IFN-beta showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-beta.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human IFN-beta.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Sample Values

Cell Culture Supernates: PBMCs were isolated from a single donor over a Ficoll-Paque PLUS density gradient. PBMCs were seeded at 5 x 106 /mL and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were left untreated or treated with 10 μg/mL of poly I:C in the presence of Lipofectamine 2000 for 24 hours. Aliquots of the cell culture supernates were removed and assayed for levels of human IFN-beta.

Condition	pg/mL
Untreated	ND
Treated	323

ND= Non-detectable

A549 adenocarcinomic human alveolar basal epithelial cells were cultured in Kaighn's Nutrient Mixture F-12 supplemented with 10% fetal bovine serum until nearly confluent. The cells were left untreated or treated with 10 μg/mL of poly I:C in the presence of Lipofectamine 2000 for 24 hours. Aliquots of the cell culture supernates were removed and assayed for levels of human IFN-beta.

Condition	pg/mL
Untreated	ND
Treated	1470

ND=Non-detectable

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision.

Cell Culture Supernates

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	20	20	10	10
Mean (pg/mL)	101	613	121	669
Standard Deviation	3.82	30.1	6.48	33.3
CV%	3.8	4.9	5.4	5.0

Recovery for Human IFN-beta Quantikine QuicKit ELISA

The recovery of human IFN-beta spiked to three levels in samples throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Serum (n=0)

Linearity

To assess the linearity of the assay, samples containing high concentrations of human IFN-beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Human IFN-beta Quantikine QuicKit ELISA

Human IFN-beta Standard Curve ELISA

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-beta

IFN-beta (Interferon-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.