Human IFN-gamma  R1/CD119 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB673F

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Detection of IFN‑ gamma  R1/CD119 in Human Blood Monocytes by Flow Cytometry.
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Human IFN-gamma  R1/CD119 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-gamma R1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse IFN‑ gamma  R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta.
Source
Monoclonal Mouse IgG1 Clone # 92101
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IFN-gamma  R1
Glu18-Gly245
Accession # P15260
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IFN‑ gamma  R1/CD119 in Human Blood Monocytes by Flow Cytometry. View Larger

Detection of IFN‑ gamma R1/CD119 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human IFN-gamma R1/CD119 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB673F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma  receptor complex is made up of two type I transmembrane proteins, IFN-gamma  R1 (IFN-gamma  R alpha ) and IFN-gamma  R2 (IFN-gamma  R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma  R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma  R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma  R1 cDNA encodes a 489 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma  R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma  R1 is constitutively expressed in most cell types. Soluble IFN-gamma  R1 that binds IFN-gamma has been detected in biological fluids.

Long Name
Interferon gamma Receptor 1
Entrez Gene IDs
3459 (Human); 15979 (Mouse)
Alternate Names
AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2

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Citation for Human IFN-gamma  R1/CD119 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CD133+ liver cancer stem cells resist interferon-gamma-induced autophagy.
    Authors: Li J, Chen J, Zeng T, He F, Chen S, Ma S, Bi J, Zhu X, Guan X
    BMC Cancer, 2016;16(1):15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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