The high-affinity IFN-gamma receptor complex is made up of two type I transmembrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 489 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids.
Human IFN‑ gamma R1/CD119 PE‑conjugated Antibody
R&D Systems | Catalog # FAB673P
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 92101
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R1
Glu18-Gly245
Accession # P15260.1
Glu18-Gly245
Accession # P15260.1
Specificity
Detects human IFN-gamma R1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse IFN‑ gamma R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IFN‑ gamma R1/CD119 PE‑conjugated Antibody
Detection of IFN‑ gamma R1/CD119 in Human Blood Monocytes by Flow Cytometry.
Human peripheral blood monocytes were stained with Mouse Anti-Human IFN-gamma R1/CD119 PE-conjugated Monoclonal Antibody (Catalog # FAB673P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human IFN‑ gamma R1/CD119 PE‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood monocytes
Sample: Human peripheral blood monocytes
Reviewed Applications
Read 1 review rated 4 using FAB673P in the following applications:
Spectra Viewer
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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: IFN-gamma R1/CD119
Long Name
Interferon gamma Receptor 1
Alternate Names
CD119, IFN-gR1, IFNgammaR1, IFNGR1
Gene Symbol
IFNGR1
UniProt
Additional IFN-gamma R1/CD119 Products
Product Documents for Human IFN‑ gamma R1/CD119 PE‑conjugated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IFN‑ gamma R1/CD119 PE‑conjugated Antibody
For research use only
Citations for Human IFN‑ gamma R1/CD119 PE‑conjugated Antibody
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Application: Flow CytometrySample Tested: See PMID: 23089549Species: MouseVerified Customer | Posted 01/27/2015
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Th1 Differentiation Pathway
