Human IFN-gamma  R1/CD119 Antibody

Catalog # Availability Size / Price Qty
MAB6731
MAB6731-SP
IFN‑ gamma  Inhibition of EMCV-induced Cytopathy and Neutralization by HumanIFN‑ gamma  R1/CD119 Antibody.
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Citations (5)
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Human IFN-gamma  R1/CD119 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-gamma R1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant mouse IFN‑ gamma  R1, recombinant human (rh) IFN-gamma R2, or rhIL-10 R beta.
Source
Monoclonal Mouse IgG1 Clone # 92101
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IFN-gamma  R1
Glu18-Gly245
Accession # P15260.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IFN-gamma  R1/CD119 (Catalog # 673-IR)
Flow Cytometry
2.5 µg/106 cells
Human whole blood monocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IFN‑ gamma  R1/CD119-mediated inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.01-0.03 µg/mL in the presence of 2 ng/mL Recombinant Human IFN‑ gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization IFN‑ gamma  Inhibition of EMCV-induced Cytopathy and Neutralization by HumanIFN‑ gamma  R1/CD119 Antibody. View Larger

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human
IFN‑ gamma  R1/CD119 Antibody.
Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse Anti-Human IFN‑ gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Human IFN‑ gamma  R1/CD119 Monoclonal Antibody (Catalog # MAB6731). The ND50 is typically 0.01-0.03 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma  receptor complex is made up of two type I membrane proteins, IFN-gamma  R1 (IFN-gamma  R alpha ) and IFN-gamma  R2 (IFN-gamma  R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% amino acid sequence identity. IFN-gamma  R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma  R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma  R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma  R1 share 52% amino acid sequence identity and bind IFN-gamma in a species-specific manner. IFN-gamma  R1 is constitutively expressed in most cell types. Soluble IFN-gamma  R1 that binds IFN-gamma has been detected in biological fluids.

References
  1. Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.
Long Name
Interferon gamma Receptor 1
Entrez Gene IDs
3459 (Human); 15979 (Mouse)
Alternate Names
AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2

Product Datasheets

Citations for Human IFN-gamma  R1/CD119 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Bispecific T cell engager (BiTE�) antibody constructs can mediate bystander tumor cell killing
    Authors: SL Ross, M Sherman, PL McElroy, JA Lofgren, G Moody, PA Baeuerle, A Coxon, T Arvedson
    PLoS ONE, 2017;12(8):e0183390.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  2. IL-1? induced HIF-1? inhibits the differentiation of human FOXP3(+) T cells
    Authors: LM Feldhoff, CM Rueda, ME Moreno-Fer, J Sauer, CM Jackson, CA Chougnet, J Rupp
    Sci Rep, 2017;7(1):465.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  3. Induction of indoleamine 2,3-dioxygenase by Borrelia burgdorferi in human immune cells correlates with pathogenic potential.
    Authors: Love A, Schwartz I, Petzke M
    J Leukoc Biol, 2015;97(2):379-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  4. Interferon-gamma differentially regulates TGF-beta1 and TGF-beta2 expression in human retinal pigment epithelial cells through JAK-STAT pathway.
    Authors: Nagineni CN, Cherukuri KS, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2007;210(1):192-200.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  5. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006;241(1):7-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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Reviews for Human IFN-gamma  R1/CD119 Antibody

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Human IFN-gamma  R1/CD119 Antibody
By Anonymous on 07/16/2018
Application: WB Sample Tested: Cancer Cells Species: Human

Cancer cells as indicated were treated with or without INF-g (10ng/ml) for 24h. Total cell lysates were subjected to western blot. PVDF membrane were probed with 1mm/ml Human Human IFN-gamma R1/CD119 Antibody (MAB6731). A specific band was detected for IFN-gamma R1/CD119 at approximately 43kDa. This experiment was conducted under reducing conditions