Human IGF-I/IGF-1 Quantikine ELISA Kit

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Human IGF-I ELISA Standard Curve
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Product Details
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Citations (89)
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Human IGF-I/IGF-1 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL)
Sensitivity
0.056 ng/mL
Assay Range
0.1 - 6 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IGF-I
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IGF-I Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-I in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IGF-I and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed IGF-I.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 0.49 1.243 2.373 0.382 0.967 1.941
Standard Deviation 0.017 0.054 0.103 0.031 0.08 0.145
CV% 3.5 4.3 4.3 8.1 8.3 7.5

Recovery

The recovery of IGF-I spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 97-102

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGF-I were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IGF-I ELISA Linearity

Data Examples

Human IGF-I ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-I/IGF-1

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.

The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.

Long Name:
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs:
3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names:
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

The conjugate must remain at 2-8 °C until use. Bring all other reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 150 µL Assay Diluent
  4.   Add 150 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL cold Conjugate
  9.   Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IGF-I/IGF-1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

89 Citations: Showing 1 - 10
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  1. Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-? and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells
    Authors: S Morscheid, A Rey-Rico, G Schmitt, H Madry, M Cucchiarin, JK Venkatesan
    Int J Mol Sci, 2019;20(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Impact of HIV-1 infection on the IGF-1 axis and angiogenic factors in pregnant Cameroonian women receiving antiretroviral therapy
    Authors: LF Esemu, EK Yuosembom, R Fang, S Rasay, BAY Fodjo, JT Nguasong, W Kidima, GL Ekali, JJ Chen, L Ndhlovu, JD Bigoga, DW Taylor, RGF Leke, A Babakhanya
    PLoS ONE, 2019;14(5):e0215825.
    Species: Human
    Sample Types: Plasma
  3. Early-Life Cadmium Exposure and Bone-Related Biomarkers: A Longitudinal Study in Children
    Authors: A Malin Igra, M Vahter, R Raqib, M Kippler
    Environ. Health Perspect., 2019;127(3):37003.
    Species: Human
    Sample Types: Plasma
  4. Human cardiac progenitor cell activation and regeneration mechanisms: exploring a novel myocardial ischemia/reperfusion in vitro model
    Authors: MJ Sebastião, M Serra, R Pereira, I Palacios, P Gomes-Alve, PM Alves
    Stem Cell Res Ther, 2019;10(1):77.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Hypoallergenic and anti-inflammatory feeds in children with complicated severe acute malnutrition: an open randomised controlled 3-arm intervention trial in Malawi
    Authors: RH Bartels, E Chimwezi, V Watson, L Pei, I Potani, B Allubha, K Chidzalo, D Wang, Q Dube, M Mallewa, A Allen, RHJ Bandsma, WP Voskuijl, SJ Allen
    Sci Rep, 2019;9(1):2304.
    Species: Human
    Sample Types: Plasma
  6. Serum concentrations of IGF-I/IGF-II as biomarkers of alcohol damage during foetal development and diagnostic markers of Foetal Alcohol Syndrome
    Authors: V Andreu-Fer, A Bastons-Co, E Navarro-Ta, S Sailer, O Garcia-Alg
    Sci Rep, 2019;9(1):1562.
    Species: Human
    Sample Types: Serum
  7. Redifferentiation of Articular Chondrocytes by Hyperacute Serum and Platelet Rich Plasma in Collagen Type I Hydrogels
    Authors: V Jeyakumar, E Niculescu-, C Bauer, Z Lacza, S Nehrer
    Int J Mol Sci, 2019;20(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Ginsenoside Rg1 Accelerates Paracrine Activity and Adipogenic Differentiation of Human Breast Adipose-Derived Stem Cells in a Dose-Dependent Manner In Vitro
    Authors: ZJ Liang, X Lu, DD Zhu, XL Yi, FX Wu, N He, C Tang, CY Wei, HM Li
    Cell Transplant, 2019;0(0):9636897198256.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures
    Authors: F Agostini, M Ruzza, D Corpillo, L Biondi, E Acquadro, B Canepa, A Viale, M Battiston, F Serra, S Aime, M Mazzucato
    PLoS ONE, 2018;13(9):e0203048.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Differences in biochemical markers between Heart-transplanted and Left Ventricular Assist Device implanted patients, during cardiac rehabilitation
    Authors: V Racca, P Castiglion, C Panzarino, M Saresella, I Marventano, A Verde, F Oliva, M Ferratini
    Sci Rep, 2018;8(1):10816.
    Species: Human
    Sample Types: Serum
  11. Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches
    Authors: C Saury, A Lardenois, C Schleder, I Leroux, B Lieubeau, L David, M Charrier, L Guével, S Viau, B Delorme, K Rouger
    Stem Cell Res Ther, 2018;9(1):128.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Characterization of induced pluripotent stem cell-derived megakaryocyte lysates for potential regenerative applications
    Authors: A Baigger, D Eicke, Y Yuzefovych, D Pogozhykh, R Blasczyk, C Figueiredo
    J. Cell. Mol. Med., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
  13. Validation of insulin-like growth factor-1 as a prognostic parameter in patients with hepatocellular carcinoma in a European cohort
    Authors: Y Huber, F Bierling, C Labenz, S Koch, I Schmidtman, R Kloeckner, S Schotten, T Huber, H Lang, MA Woerns, PR Galle, A Weinmann, J Weinmann-M
    BMC Cancer, 2018;18(1):774.
    Species: Human
    Sample Types: Serum
  14. Matrine induces senescence of human glioblastoma cells through suppression of the IGF1/PI3K/AKT/p27 signaling pathway
    Authors: W Zhou, J Wang, Q Qi, Z Feng, B Huang, A Chen, D Zhang, W Li, Q Zhang, R Bjerkvig, X Li, J Wang
    Cancer Med, 2018;0(0):.
    Species: Human
    Sample Types: Cell Cultue Supernates
  15. Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth
    Authors: DH Bak, MJ Choi, SR Kim, BC Lee, JM Kim, ES Jeon, W Oh, ES Lim, BC Park, MJ Kim, J Na, BJ Kim
    Korean J. Physiol. Pharmacol., 2018;22(5):555-566.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. The effect of autologous platelet rich plasma on tenocytes of the human rotator cuff
    Authors: S Pauly, F Klatte-Sch, K Stahnke, M Scheibel, B Wildemann
    BMC Musculoskelet Disord, 2018;19(1):422.
    Species: Human
    Sample Types: Plasma
  17. A 2-Week Specific Volleyball Training Supported by the Whole Body Cryostimulation Protocol Induced an Increase of Growth Factors and Counteracted Deterioration of Physical Performance
    Authors: J Jaworska, K Micielska, M Koz?owska, K Wnorowski, J Skrobecki, L Radzimi?sk, A Babi?ska, E Rodziewicz, G Lombardi, E Ziemann
    Front Physiol, 2018;9(0):1711.
    Species: Human
    Sample Types: Serum
  18. The effects of a combined bodyweight-based and elastic bands resistance training, with or without protein supplementation, on muscle mass, signaling and heat shock response in healthy older people
    Authors: M Krause, D Crognale, K Cogan, S Contarelli, B Egan, P Newsholme, G De Vito
    Exp. Gerontol., 2018;115(0):104-113.
    Species: Human
    Sample Types: Plasma
  19. Physical Exercise and Spatial Training: A Longitudinal Study of Effects on Cognition, Growth Factors, and Hippocampal Plasticity
    Authors: L Woost, PL Bazin, M Taubert, R Trampel, CL Tardif, A Garthe, G Kempermann, U Renner, G Stalla, DVM Ott, V Rjosk, H Obrig, A Villringer, E Roggenhofe, TA Klein
    Sci Rep, 2018;8(1):4239.
    Species: Human
    Sample Types: Serum
  20. Relationships of Age and Sex with Cytokine Content and Distribution in Human Platelet Fibrin Gels
    Authors: MY Bai, MH Chuang, MF Lin, SL Tang, CC Wong, WP Chan
    Sci Rep, 2018;8(1):10642.
    Species: Rabbit
    Sample Types: Platelet Rich Fibrin
  21. Impact of Chronic Neonatal Intermittent Hypoxia on Severity of Retinal Damage in a Rat Model of Oxygen-Induced Retinopathy
    Authors: KD Beharry, CL Cai, T Ahmad, S Guzel, GB Valencia, JV Aranda
    J Nat Sci, 2018;4(3):.
    Species: Rat
    Sample Types: Tissue Homogenates
  22. Evaluation of Coated Steroidal Implants Containing Trenbolone Acetate and Estradiol-17? on Live Performance, Carcass Traits, and Sera Metabolites in Finishing Steers
    Authors: ZK Smith, AJ Thompson, JP Hutcheson, WT Nichols, BJ Johnson
    J. Anim. Sci., 2018;0(0):.
    Species: Bovine
    Sample Types: Serum
  23. Hypoxic preconditioning of human cardiosphere-derived cell sheets enhances cellular functions via activation of the PI3K/Akt/mTOR/HIF-1? pathway
    Authors: Y Tanaka, T Hosoyama, A Mikamo, H Kurazumi, A Nishimoto, K Ueno, B Shirasawa, K Hamano
    Am J Transl Res, 2017;9(2):664-673.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. A phase II randomized, double-blind, presurgical trial of Polyphenon E in bladder cancer patients to evaluate pharmacodynamics and bladder tissue biomarkers
    Authors: JR Gee, DR Saltzstein, K Kim, JM Kolesar, W Huang, TC Havighurst, BW Wollmer, J Stublaski, T Downs, H Mukhtar, M House, HL Parnes, HH Bailey
    Cancer Prev Res (Phila), 2017;0(0):.
    Species: Human
    Sample Types: Serum
  25. Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy
    Authors: K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya
    Sci Rep, 2017;7(1):8484.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Evaluation of Not-Activated and Activated PRP in Hair Loss Treatment: Role of Growth Factor and Cytokine Concentrations Obtained by Different Collection Systems
    Authors: P Gentile, JP Cole, MA Cole, S Garcovich, A Bielli, MG Scioli, A Orlandi, C Insalaco, V Cervelli
    Int J Mol Sci, 2017;18(2):.
    Species: Human
    Sample Types: Plasma
  27. Evaluation of autologous skeletal muscle-derived factors for regenerative medicine applications
    Authors: M Yoshikawa, T Nakasa, M Ishikawa, N Adachi, M Ochi
    Bone Joint Res, 2017;6(5):277-283.
    Species: Human
    Sample Types: Tissue Homogenates
  28. Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes
    Authors: J Frisch, P Orth, A Rey-Rico, JK Venkatesan, G Schmitt, H Madry, D Kohn, M Cucchiarin
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Effect of Physical Activity on Cognitive Development: Protocol for a 15-Year Longitudinal Follow-Up Study
    Authors: G Zhao, M Quan, L Su, H Zhang, J Zhang, J Zhang, H Fang, ZB Cao, Z Zhu, Z Niu, R Wang, P Chen
    Biomed Res Int, 2017;2017(0):8568459.
    Species: Human
    Sample Types: Plasma
  30. Temporal changes in liver tissue metabolome of lambs fed low-quality roughage
    Authors: W Xu, N Okayama, A Iwasawa, M Yayota
    Anim. Sci. J., 2017;0(0):.
    Species: Ovine
    Sample Types: Serum
  31. Metformin inhibits the proliferation of benign prostatic epithelial cells
    Authors: Z Wang, X Xiao, R Ge, J Li, CW Johnson, C Rassoulian, AF Olumi
    PLoS ONE, 2017;12(3):e0173335.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Angiogenesis in newly regenerated bone by secretomes of human mesenchymal stem cells
    Authors: W Katagiri, T Kawai, M Osugi, Y Sugimura-W, K Sakaguchi, T Kojima, T Kobayashi
    Maxillofac Plast Reconstr Surg, 2017;39(1):8.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Relationships of insulin-like growth factor-1, its binding proteins, and cardiometabolic risk in hypertensive perimenopausal women
    Authors: A Olszanecka, A Dragan, K Kawecka-Ja, D Fedak, D Czarnecka
    Metab. Clin. Exp., 2017;69(0):96-106.
    Species: Human
    Sample Types: Plasma
  34. Concurrent Aerobic and Resistance Training Has Anti-Inflammatory Effects and Increases Both Plasma and Leukocyte Levels of IGF-1 in Late Middle-Aged Type 2 Diabetic Patients
    Authors: G Annibalini, F Lucertini, D Agostini, L Vallorani, A Gioacchini, E Barbieri, M Guescini, L Casadei, A Passalia, M Del Sal, G Piccoli, M Andreani, A Federici, V Stocchi
    Oxid Med Cell Longev, 2017;2017(0):3937842.
    Species: Human
    Sample Types: Plasma
  35. Effects of transdermal testosterone gel or an aromatase inhibitor on serum concentration and pulsatility of growth hormone in older men with age-related low testosterone
    Authors: JP Dias, JD Veldhuis, O Carlson, M Shardell, CW Chia, D Melvin, JM Egan, S Basaria
    Metab. Clin. Exp., 2017;69(0):143-147.
    Species: Human
    Sample Types: Serum
  36. Prenatal exposure to tobacco smoke leads to increased mitochondrial DNA content in umbilical cord serum associated to reduced gestational age
    Authors: Francesca Pirini
    Int J Environ Health Res, 2017;27(1):52-67.
    Species: Human
    Sample Types: Serum
  37. Serum IGF-1 is associated with cognitive functions in early, drug-na�ve Parkinson's disease
    Authors: M Picillo, R Pivonello, G Santangelo, C Pivonello, R Savastano, R Auriemma, M Amboni, S Scannapiec, A Pierro, A Colao, P Barone, MT Pellecchia
    PLoS ONE, 2017;12(10):e0186508.
    Species: Human
    Sample Types: Serum
  38. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
    Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
    J Transl Med, 2017;15(1):90.
    Species: Human
    Sample Types: Cell Lysates
  39. Five-weekly S-1 plus cisplatin therapy combined with trastuzumab therapy in HER2-positive gastric cancer: a phase II trial and biomarker study (WJOG7212G)
    Authors: Y Miura, Y Sukawa, S Hironaka, M Mori, K Nishikawa, S Tokunaga, H Okuda, T Sakamoto, K Taku, K Nishikawa, T Moriwaki, Y Negoro, Y Kimura, K Uchino, K Shinozaki, H Shinozaki, N Musha, H Yoshiyama, T Tsuda, Y Miyata, N Sugimoto, T Shirakawa, M Ito, K Yonesaka, K Yoshimura, N Boku, K Nosho, T Takano, I Hyodo
    Gastric Cancer, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  40. High glycemic index diet in PCOS patients. The analysis of IGF I and TNF-? pathways in metabolic disorders
    Authors: M Szczuko
    Med. Hypotheses, 2016;96(0):42-47.
    Species: Human
    Sample Types: Plasma
  41. The tobacco-specific carcinogen-operated calcium channel promotes lung tumorigenesis via IGF2 exocytosis in lung epithelial cells
    Nat Commun, 2016;7(0):12961.
    Species: Human
    Sample Types: Cell Culture Supernates
  42. Impact of Hyperglycemia and Low Oxygen Tension on Adipose-Derived Stem Cells Compared with Dermal Fibroblasts and Keratinocytes: Importance for Wound Healing in Type 2 Diabetes
    PLoS ONE, 2016;11(12):e0168058.
    Species: Human
    Sample Types: Cell Culture Supernates
  43. Phase IIa, randomized placebo-controlled trial of single high dose cholecalciferol (vitamin D3) and daily Genistein (G-2535) versus double placebo in men with early stage prostate cancer undergoing prostatectomy
    Authors: Howard H Bailey
    Am J Clin Exp Urol, 2016;4(2):17-27.
    Species: Human
    Sample Types: Serum
  44. Genetic variation in IGF1 predicts renal cell carcinoma susceptibility and prognosis in Chinese population
    Sci Rep, 2016;6(0):39014.
    Species: Human
    Sample Types: Serum
  45. A preliminary study of pamidronic acid downregulation of angiogenic factors IGF-1/PECAM-1 expression in circulating level in bone metastatic breast cancer patients
    Authors: Zeng Wang
    Onco Targets Ther, 2016;9(0):3147-52.
    Species: Human
    Sample Types: Serum
  46. Neovascularization Potential of Blood Outgrowth Endothelial Cells From Patients With Stable Ischemic Heart Failure Is Preserved
    Authors: D Dauwe, B Pelacho, A Wibowo, AS Walravens, K Verdonck, H Gillijns, E Caluwe, P Pokreisz, N van Gastel, G Carmeliet, M Depypere, F Maes, N Vanden Dri, W Droogne, J Van Cleemp, J Vanhaecke, F Prosper, C Verfaillie, A Luttun, S Janssens
    J Am Heart Assoc, 2016;5(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  47. Biological effects of plasma rich in growth factors (PRGF) on human endometrial fibroblasts
    Authors: Gorka Orive
    Eur. J. Obstet. Gynecol. Reprod. Biol., 2016;206(0):125-130.
    Species: Human
    Sample Types: Plasma
  48. Adipose-derived mesenchymal stem cells from patients with atherosclerotic renovascular disease have increased DNA damage and reduced angiogenesis that can be modified by hypoxia
    Stem Cell Res Ther, 2016;7(1):128.
    Species: Human
    Sample Types: Cell Culture Supernates
  49. Randomized Trial Testing the Effects of Eating Frequency on Two Hormonal Biomarkers of Metabolism and Energy Balance
    Authors: Martine M Perrigue
    Nutr Cancer, 2016;0(0):1-8.
    Species: Human
    Sample Types: Plasma
  50. An adverse immune-endocrine profile in patients with tuberculosis and type 2 diabetes
    Authors: O Bottasso
    Tuberculosis (Edinb), 2016;101(0):95-101.
    Species: Human
    Sample Types: Plasma
  51. Overcoming the bottleneck of platelet lysate supply in large-scale clinical expansion of adipose-derived stem cells: A comparison of fresh versus three types of platelet lysates from outdated buffy coat-derived platelet concentrates
    Authors: Anne Fischer-Ni
    Cytotherapy, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  52. IGF1R as a Key Target in High Risk, Metastatic Medulloblastoma
    Authors: Matthew N Svalina
    Sci Rep, 2016;6(0):27012.
    Species: Human
    Sample Types: CSF
  53. Effects of exercise and milk fat globule membrane (MFGM) supplementation on body composition, physical function, and hematological parameters in community-dwelling frail Japanese women: a randomized double blind, placebo-controlled, follow-up trial.
    Authors: Kim H, Suzuki T, Kim M, Kojima N, Ota N, Shimotoyodome A, Hase T, Hosoi E, Yoshida H
    PLoS ONE, 2015;10(2):e0116256.
    Species: Human
    Sample Types: Serum
  54. Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I
    Authors: Magali Cucchiarin
    Tissue Eng Part A, 2015;21(17):2460-71.
    Species: Human
    Sample Types: Cell Culture Supernates
  55. LMP1 promotes expression of insulin-like growth factor 1 (IGF1) to selectively activate IGF1 receptor and drive cell proliferation.
    Authors: Tworkoski K, Raab-Traub N
    J Virol, 2015;89(5):2590-602.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. Implantation of rAAV5-IGF-I transduced autologous chondrocytes improves cartilage repair in full-thickness defects in the equine model.
    Authors: Ortved K, Begum L, Mohammed H, Nixon A
    Mol Ther, 2015;23(2):363-73.
    Species: Equine
    Sample Types: Synovial Fluid
  57. Overexpression of human IGF-I via direct rAAV-mediated gene transfer improves the early repair of articular cartilage defects in vivo.
    Authors: Cucchiarini M, Madry H
    Gene Ther, 2014;21(9):811-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  58. A randomized phase II presurgical trial of transdermal 4-hydroxytamoxifen gel versus oral tamoxifen in women with ductal carcinoma in situ of the breast.
    Authors: Lee O, Page K, Ivancic D, Helenowski I, Parini V, Sullivan M, Margenthaler J, Chatterton R, Jovanovic B, Dunn B, Heckman-Stoddard B, Foster K, Muzzio M, Shklovskaya J, Skripkauskas S, Kulesza P, Green D, Hansen N, Bethke K, Jeruss J, Bergan R, Khan S
    Clin Cancer Res, 2014;20(14):3672-82.
    Species: Human
    Sample Types: Plasma
  59. Arsenic exposure affects plasma insulin-like growth factor 1 (IGF-1) in children in rural Bangladesh.
    Authors: Ahmed S, Rekha R, Ahsan K, Doi M, Grander M, Roy A, Ekstrom E, Wagatsuma Y, Vahter M, Raqib R
    PLoS ONE, 2013;8(11):e81530.
    Species: Human
    Sample Types: Plasma
  60. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis.
    Authors: Ho I, Toh H, Ng W, Teo Y, Guo C, Hui K, Lam P
    Stem Cells, 2013;31(1):146-55.
    Species: Human
    Sample Types: Tissue Homogenates
  61. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
    Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
    BMC Cell Biol, 2013;14(0):48.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Role of serum concentration of VEGFR1 and TIMP2 on clinical outcome in primary cervical cancer: results of a companion protocol of the randomized, NOGGO-AGO phase III adjuvant trial of simultaneous cisplatin-based radiochemotherapy vs. carboplatin and paclitaxel containing sequential radiotherapy.
    Authors: Braicu E, Fotopoulou C, Chekerov R, Richter R, Blohmer J, Kummel S, Stamatian F, Yalcinkaya I, Mentze M, Lichtenegger W, Sehouli J
    Cytokine, 2013;61(3):755-8.
    Species: Human
    Sample Types: Serum
  63. Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis.
    Authors: Weimer A, Madry H, Venkatesan JK
    Mol. Med., 2012;18(1):346-58.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. Maternal and fetal placental growth hormone and IGF axis in type 1 diabetic pregnancy.
    Authors: Higgins MF, Russell NE, Crossey PA, Nyhan KC, Brazil DP, McAuliffe FM
    PLoS ONE, 2012;7(2):e29164.
    Species: Human
    Sample Types: Plasma
  65. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
    Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
    ALTEX, 2011;28(4):305-16.
    Species: Human
    Sample Types: Cell Lysates
  66. Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
    Authors: Li TS, Cheng K, Malliaras K, Matsushita N, Sun B, Marban L, Zhang Y, Marban E
    Cardiovasc. Res., 2011;89(1):157-65.
    Species: Human
    Sample Types: Cell Culture Supernates
  67. Inflammation associated with obesity: relationship with blood and bone marrow endothelial cells.
    Authors: McGuire TR, Brusnahan SK, Bilek LD, Jackson JD, Kessinger MA, Berger AM, Garvin KL, O'Kane BJ, Tuljapurkar SR, Sharp JG
    Obesity (Silver Spring), 2011;19(11):2130-6.
    Species: Human
    Sample Types: Plasma
  68. The stature of boys is inversely correlated to the levels of their sertoli cell hormones: do the testes restrain the maturation of boys?
    Authors: Morgan K, Dennis NA, Ruffman T, Bilkey DK, McLennan IS
    PLoS ONE, 2011;6(6):e20533.
    Species: Human
    Sample Types: Serum
  69. Association of angiopoietin-2, C-reactive protein and markers of obesity and insulin resistance with survival outcome in colorectal cancer.
    Authors: Volkova E, Willis JA, Wells JE, Robinson BA, Dachs GU, Currie MJ
    Br. J. Cancer, 2011;104(1):51-9.
    Species: Human
    Sample Types: Serum
  70. Prognostic significance of angiogenic factors in uterine cervical cancer.
    Authors: Landt S, Wehling M, Heidecke H, Jeschke S, Korlach S, Stoblen F, Schmid P, Blohmer JU, Lichtenegger W, Sehouli J, Kummel S
    Anticancer Res., 2011;31(8):2589-95.
    Species: Human
    Sample Types: Serum
  71. Abnormalities in metalloproteinase pathways and IGF-I axis: a link between birth weight, hypertension, and vascular damage in childhood.
    Authors: Sesso R, Franco MC
    Am. J. Hypertens., 2010;23(1):6-11.
    Species: Human
    Sample Types: Plasma
  72. Insulin-like growth factor-1 and neurotrophin-3 gene therapy prevents motor decline in an X-linked adrenoleukodystrophy mouse model.
    Authors: Mastroeni R, Bensadoun JC, Charvin D, Aebischer P, Pujol A, Raoul C
    Ann. Neurol., 2009;66(1):117-22.
    Species: Human
    Sample Types: CSF
  73. Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells.
    Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD
    Cancer Res., 2009;69(7):2996-3003.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. cAMP/PKA pathway activation in human mesenchymal stem cells in vitro results in robust bone formation in vivo.
    Authors: Siddappa R, Martens A, Doorn J, Leusink A, Olivo C, Licht R, van Rijn L, Gaspar C, Fodde R, Janssen F, van Blitterswijk C, de Boer J
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(20):7281-6.
    Species: Human
    Sample Types: Cell Culture Supernates
  75. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  76. Identification of transforming growth factor beta1-driven genetic programs of acute lung fibrosis.
    Authors: Pulichino AM, Wang IM, Caron A, Mortimer J, Auger A, Boie Y, Elias JA, Kartono A, Xu L, Menetski J, Sayegh CE
    Am. J. Respir. Cell Mol. Biol., 2008;39(3):324-36.
    Species: Human
    Sample Types: BALF
  77. Insulin-like growth factor-1 improves survival in sepsis via enhanced hepatic bacterial clearance.
    Authors: Ashare A, Nymon AB, Doerschug KC, Morrison JM, Monick MM, Hunninghake GW
    Am. J. Respir. Crit. Care Med., 2008;178(2):149-57.
    Species: Human
    Sample Types: Serum
  78. The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection.
    Authors: Asimakopoulos B, Abu-Hassan D, Metzen E, Al-Hasani S, Diedrich K, Nikolettos N
    Fertil. Steril., 2007;90(1):60-4.
    Species: Human
    Sample Types: Follicular Fluid
  79. Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy.
    Authors: Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A
    Clin. Cancer Res., 2007;13(3):1000-9.
    Species: Human
    Sample Types: Plasma
  80. Serum insulin-like growth factor I (IGF-I) and IGF-binding protein 3 (IGFBP-3) in IVF patients with polycystic ovary syndrome: correlations with outcome.
    Authors: Schoyer KD, Liu HC, Witkin S, Rosenwaks Z, Spandorfer SD
    Fertil. Steril., 2007;88(1):139-44.
    Species: Human
    Sample Types: Serum
  81. Muscle atrophy and hypertrophy signaling in patients with chronic obstructive pulmonary disease.
    Authors: Doucet M, Russell AP, Leger B, Debigare R, Joanisse DR, Caron MA, LeBlanc P, Maltais F
    Am. J. Respir. Crit. Care Med., 2007;176(3):261-9.
    Species: Human
    Sample Types: Plasma
  82. Peripheral elevation of IGF-1 fails to alter Abeta clearance in multiple in vivo models.
    Authors: Lanz TA, Salatto CT, Semproni AR, Marconi M, Brown TM, Richter KE, Schmidt K, Nelson FR, Schachter JB
    Biochem. Pharmacol., 2007;75(5):1093-103.
    Species: Canine
    Sample Types: Plasma
  83. Serum concentrations of insulin-like growth factor-I (igf-I) as a marker of liver fibrosis in patients with chronic hepatitis C.
    Authors: Lorenzo-Zuniga V, Bartoli R, Masnou H, Montoliu S, Morillas RM, Planas R
    Dig. Dis. Sci., 2007;52(11):3245-50.
    Species: Human
    Sample Types: Serum
  84. Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo development.
    Authors: Wang TH, Chang CL, Wu HM, Chiu YM, Chen CK, Wang HS
    Fertil. Steril., 2006;86(5):1392-401.
    Species: Human
    Sample Types: Follicular Fluid
  85. Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells.
    Authors: Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB
    Glia, 2006;53(8):845-57.
    Species: Human
    Sample Types: Cell Culture Supernates
  86. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
    Authors: Herrmann S, Green J, Weber T, Scharfenberger A
    Bone, 2006;39(5):1156-63.
    Species: Human
    Sample Types: Tissue Homogenates
  87. Growth hormone-induced stimulation of multilineage human hematopoiesis.
    Authors: Hanley MB, Napolitano LA, McCune JM
    Stem Cells, 2005;23(8):1170-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  88. Insulin growth factor-1 protects against excitotoxicity in the rat striatum.
    Authors: Escartin C, Boyer F, Bemelmans AP, Hantraye P, Brouillet E
    Neuroreport, 2004;15(14):2251-4.
    Species: Human
    Sample Types: Recombinant Protein
  89. Dose response of bone mass to dietary arachidonic acid in piglets fed cow milk-based formula.
    Authors: Blanaru JL, Kohut JR, Fitzpatrick-Wong SC, Weiler HA
    Am. J. Clin. Nutr., 2004;79(1):139-47.
    Species: Porcine
    Sample Types: Plasma

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Human IGF-I/IGF-1 Quantikine ELISA Kit
By Anonymous on 06/19/2019
Sample Tested: Human Serum

Human IGF-I/IGF-1 Quantikine ELISA Kit
By Anonymous on 05/24/2019
Sample Tested: Plasma

Human IGF-I/IGF-1 Quantikine ELISA Kit
By Anonymous on 01/10/2019
Sample Tested: Serum

Human IGF-I Quantikine ELISA Kit
By Anonymous on 03/31/2018
Sample Tested: Serum

Human IGF-I Quantikine ELISA Kit
By Anonymous on 03/22/2018
Sample Tested: blood products,Recombinant protein

Human IGF-I Quantikine ELISA Kit
By Anonymous on 12/01/2017
Sample Tested: EDTA Plasma

Human IGF-I Quantikine ELISA Kit
By Anonymous on 12/01/2017
Sample Tested: Serum

Human IGF-I Quantikine ELISA Kit
By Anonymous on 09/27/2017
Sample Tested: Plasma

Human IGF-I Quantikine ELISA Kit
By Anonymous on 05/16/2017
Sample Tested: Human plasma

Human IGF-I Quantikine ELISA Kit
By Anonymous on 05/09/2017
Sample Tested: human serum

Human IGF-I Quantikine ELISA Kit
By Anonymous on 11/17/2016

Human IGF-I Quantikine ELISA Kit
By Anonymous on 10/24/2016
Sample Tested: Serum

The values are always stable.


Human IGF-I Quantikine ELISA Kit
By Anonymous on 10/14/2016
Sample Tested: Cell Culture Supernates

Human IGF-I Quantikine ELISA Kit
By Anonymous on 07/12/2016
Sample Tested: Cartilage tissue