Human IGF-II/IGF2 Quantikine ELISA Kit

R&D Systems | Catalog # DG200

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Key Product Details

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Serum-free Cell Culture Supernates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (25 µL)

Sensitivity

5.41 pg/mL

Assay Range

12.5-800 pg/mL (Serum-free Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Human IGF-II/IGF2 Quantikine ELISA Kit

The Quantikine® Human IGF-II Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-II in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human IGF-II and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-II showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGF-II.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human IGF-II.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

EDTA Plasma, Heparin Plasma, Serum, Serum-free Cell Culture Supernates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 128 305 566 136 307 558
Standard Deviation 4.05 7.20 15.6 9.89 28.5 28.2
CV% 3.2 2.4 2.8 7.3 9.3 5.1

Recovery for Human IGF-II/IGF2 Quantikine ELISA Kit

The recovery of human IGF-II spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum-free Cell Culture Media (n=4) 95 82-104
Urine (n=4) 94 79-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IGF-II were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Human IGF-II ELISA Linearity

Scientific Data Images for Human IGF-II/IGF2 Quantikine ELISA Kit

Human IGF-II ELISA Standard Curve

Human IGF-II ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-II/IGF2

IGF-II/IGF2 (Insulin-like growth factor II; also multiplication-stimulating polypeptide/MSP and somatomedin-A) belongs to the insulin family of peptide growth factors. It is part of a complex system of growth and metabolic-regulating proteins that is particularly important during development. It has been associated with nervous system proliferation and differentiation, myelination, adrenal cortical proliferation, and skeletal growth and differentiation. In human, IGF2 is primarily synthesized by the liver, and circulates at high levels in both fetus and adult. In rodent, however, IGF2 levels drop after the perinatal period. IGF2 is produced by astrocytes, hepatocytes, osteoblasts, embryonic striated muscle cells plus Kupffer cells and Ito cells. IGF2 binds to IGF1R, Insulin R (IR)-type A, IGF1R:Insulin R-A hybrids, IGF2R, and IGF binding proteins 1-6. The first three receptors initiate downstream signaling events, the IGF2R sequesters local IGF2, and the six IGFBPs regulate IGF2 activity in various tissues.

Long Name

Insulin-like Growth Factor II

Alternate Names

C11orf43, GRDF, IGF-2, IGF2, IGFII, MSA, PEG2, PP9974

Entrez Gene IDs

3481 (Human); 16002 (Mouse)

Gene Symbol

IGF2

Additional IGF-II/IGF2 Products

Product Documents for Human IGF-II/IGF2 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IGF-II/IGF2 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IGF-II/IGF2 Quantikine ELISA Kit

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