Human IGSF3 Biotinylated Antibody

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Product Details
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Human IGSF3 Biotinylated Antibody Summary

Species Reactivity
Detects human IGSF3 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) IGSF4B, rhIGSF4C, and rhIGSF8 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human IGSF3
Accession # AAI10652
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human IGSF3

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF3

IGSF3 (Immunoglobulin superfamily member 3; also EWI-3) is a 133 kDa (predicted) member of the EWI subfamily of the Ig-Superfamily of molecules. It is reported to be widely expressed, with concentration in placenta and lung. Human IGSF3 an 1194 amino acid (aa) type I transmembrane protein that contains a 19 aa signal sequence followed by an 1175 aa mature region (aa 20-1194) (SwissProt # O75054). The molecule's extracellular region is 1105 aa in length (aa 20-1124), and contains eight C2-type Ig-like domains (aa 22-1097). This region also possesses at least two utilized phosphorylation sites at Thr617and Ser625, plus an EWI motif in the second Ig-like domain. IGSF3 likely exists as a disulfide-linked homodimer. There are two potential isoform variants. One contains a 20 aa insertion after Pro406, while another shows the same insert coupled to a premature truncation after Pro833. GenBank Accession # AAI10652 is identical to the SwissProt sequence above save for a double Glu substitution for Asp1020 in the eighth Ig-like domain. Over aa 20‑1125, human IGSF3 shares 92% aa identity with mouse IGSF3.

Long Name
Immunoglobulin Superfamily, Member 3
Entrez Gene IDs
3321 (Human); 78908 (Mouse); 295325 (Rat)
Alternate Names
EWI-3; glu-Trp-Ile EWI motif-containing protein 3; IGSF3; immunoglobulin superfamily, member 3; member 3; V8

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