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Human IL-10 QuicKit ELISA

Name: Human IL-10 QuicKit ELISA
Brand: R&D Systems
SKU: QK1064
Price: 720 USD
Availability: InStock

R&D Systems | Catalog # QK1064

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

80 minutes

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity

1.65 pg/mL

Assay Range

37.5-2400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

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Product Summary for Human IL-10 QuicKit ELISA

The Quantikine QuicKit Human IL-10 Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human IL-10 levels in cell culture supernates, serum, and plasma.It contains recombinant human IL-10 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human IL-10 showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-10.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human IL-10.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	20	20	10	10
Mean (pg/mL)	274	1317	272	1334
Standard Deviation	7.91	49.9	27.8	100
CV%	2.9	3.8	10.2	7.5

Recovery for Human IL-10 QuicKit ELISA

The recovery of IL-10 is spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	96	87-105
EDTA Plasma (n=2)	84	75-90
Heparin Plasma (n=2)	78	65-97
Serum (n=2)	92	80-105

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-10 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Human IL-10 QuicKit ELISA

Human IL-10 Quantikine QuicKit ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-10

IL-10 (Interleukin-10) functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 100034187 (Equine); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)