Human IL‑11 R alpha Alexa Fluor™ Plus 647‑conjugated Antibody

R&D Systems | Catalog # FAB19771AFP647

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 1020854
View all IL-11 R alpha Primary Antibodies »

Product Specifications

Specificity

Detects human IL-11 R alpha in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human IL‑11 R alpha Alexa Fluor™ Plus 647‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IL-11 R alpha

Interleukin-11 receptor alpha (IL-11 R alpha ) is a 49 kDa type I transmembrane member of the gp130 subfamily of the hematopoietic cytokine receptor family (1-3). Mature human IL-11 R alpha consists of a 347 amino acid (aa) extracellular domain (ECD) that contains a C2 type Ig-like domain, two fibronectin type III domains, two potential glycosylation sites, and a WSxWS motif, followed by a 21 aa transmembrane region and a 31 aa cytoplasmic domain (4). Within the ECD, human IL-11 R alpha shares 84%, 82%, 90%, and 86% aa sequence identity with mouse, rat, equine, and bovine IL-11 R alpha, respectively. Alternative splicing generates an additional isoform that lacks the cytoplasmic domain (4). Upon low affinity binding to IL-11, IL-11 R alpha associates with gp130 to form a high affinity receptor complex (1, 3). gp130 also functions as a subunit in the receptors for Cardiotrophin-1, CNTF, IL-6, IL-27, IL-31, LIF, and Oncostatin M (5). IL-11 R alpha is widely expressed in adults, embryos, and embryonic stem cells (4, 6, 7). Deletion of IL-11 R alpha in female mice causes faulty decidualization, lack of decidual NK cells, and infertility (8-10). IL-11 is anti-apoptotic for oligodendrocytes, and lack of IL-11 R alpha increases the severity of experimental autoimmune encephalitis (11, 12). IL-11 R alpha is also anti-apoptotic for colonic epithelia, and increased IL-11 signaling may be a factor in inflammation-associated gastrointestinal cancer development (3, 13). IL-11 R alpha enhances osteoclast differentiation and bone remodeling but inhibits adipocyte differentiation (1, 2). Recombinant soluble IL-11 R alpha confers IL-11 responsiveness to cells expressing gp130, while in cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha acts as an IL-11 antagonist (14-16).

References

  1. Sims, N.A. and N.C. Walsh (2010) BMB Reports 43:513.
  2. White, U.A. and J.M. Stephens (2011) Curr. Pharm. Des. 17:340.
  3. Kiessling, S. et al. (2004) J. Biol. Chem. 279:10304.
  4. Cherel, M. et al. (1995) Blood 86:2534.
  5. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
  6. Hilton, D.J. et al. (1994) EMBO J. 13:4765.
  7. Davidson, A.J. et al. (1997) Stem Cells 15:119.
  8. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  9. Robb, L. et al. (1998) Nat. Med. 4:303.
  10. Ain, R. et al. (2004) Dev. Dyn. 231:700.
  11. Gurfein, B.T. et al. (2009) J. Immunol. 183:4229.
  12. Zhang, J. et al. (2011) J. Immunol. 187:1129.
  13. Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
  14. Curtis, D.J. et al. (1997) Blood 90:4403.
  15. Baumann, H. et al. (1996) J. Immunol. 157:284.
  16. Karow, J. et al. (1996) Biochem. J. 318:489.

Long Name

Interleukin 11 Receptor alpha

Alternate Names

IL-11Ra, IL11R alpha, IL11RA

Entrez Gene IDs

3590 (Human); 16157 (Mouse)

Gene Symbol

IL11RA

UniProt

Q14626

Additional IL-11 R alpha Products

Product Documents for Human IL‑11 R alpha Alexa Fluor™ Plus 647‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL‑11 R alpha Alexa Fluor™ Plus 647‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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