Human IL-12 R beta 1 Fluorescein-conjugated Antibody
Human IL-12 R beta 1 Fluorescein-conjugated Antibody Summary
Accession # P42701
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑12 R beta 1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 5 µg/mL PHA for 48 hours (B) untreated, were stained with Mouse Anti-Human IL-12 R beta 1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB839F) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-12 R beta 1
Interleukin12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta 1 and IL-12 R beta 2. Individually, IL-12 R beta 1 and IL-12 R beta 2 bind IL‑12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I transmembrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 1 cDNA encodes a 662 amino acid (aa) protein with a putative 23 aa signal peptide that is cleaved to generate the mature protein with a 522 aa extracellular domain, a 25 aa transmembrane domain and a 92 aa cytoplasmic region. Expression of IL-12 R beta 1 is detected in activated T cells, NK cells and B cells. The expression of IL‑12 R beta 2 is more restricted and appears to be limited to Th2 cells.
Citation for Human IL-12 R beta 1 Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity
Authors: Lars Fugger
Sci Transl Med, 2016-11-02;8(363):363ra149.
Sample Types: Whole Cells
Applications: Flow Cytometry
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