Human IL-12 R beta 1 PE-conjugated Antibody

(3 citations)   
  • Species Reactivity
  • Specificity
    Detects human IL-12 R beta 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-12 R beta 1 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 69310
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 1
    Accession # P42701
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑12 R beta 1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 5 μg/mL PHA for 48 hours  or (B) untreated were stained with Mouse Anti-Human IL‑12 R beta 1 PE‑conjugated Monoclonal Antibody (Catalog # FAB839P) and Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-12 R beta 1

Interleukin12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta 1 and IL-12 R beta 2. Individually, IL-12 R beta 1 and IL-12 R beta 2 bind IL‑12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I transmembrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 1 cDNA encodes a 662 amino acid (aa) protein with a putative 23 aa signal peptide that is cleaved to generate the mature protein with a 522 aa extracellular domain, a 25 aa transmembrane domain and a 92 aa cytoplasmic region. Expression of IL-12 R beta 1 is detected in activated T cells, NK cells and B cells. The expression of IL‑12 R beta 2 is more restricted and appears to be limited to Th2 cells.

  • References:
    1. Gately, M.K. et al. (1998) Annu. Rev. Immunol. 16:495.
  • Long Name:
    Interleukin 12 Receptor beta 1
  • Entrez Gene IDs:
    3594 (Human); 16161 (Mouse)
  • Alternate Names:
    CD212 antigen; CD212; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R beta 1; IL-12R subunit beta-1; IL12R; IL12RB; IL12RB1; IL-12Rb1; IL-12R-BETA1; IL-12R-beta-1; interleukin 12 receptor, beta 1; interleukin-12 receptor beta-1 chain; interleukin-12 receptor subunit beta-1; MGC34454
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo.
    Authors: Cocco C, Di Carlo E, Zupo S
    Leukemia, 2012;26(6):1365-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. IL-21 in the bone marrow microenvironment contributes to IgM secretion and proliferation of malignant cells in Waldenstrom macroglobulinemia.
    Authors: Hodge, Lucy S, Ziesmer, Steve C, Yang, Zhi Zhan, Secreto, Frank J, Gertz, Morie A, Novak, Anne J, Ansell, Stephen
    Blood, 2012;120(18):3774-82.
    Species: Human
    Sample Type: whole cells
    Application: Flow
  3. A novel form of complete IL-12/IL-23 receptor beta1 deficiency with cell surface-expressed nonfunctional receptors.
    Authors: Fieschi C, Bosticardo M, de Beaucoudrey L, Boisson-Dupuis S, Feinberg J, Santos OF, Bustamante J, Levy J, Candotti F, Casanova JL
    Blood, 2004;104(7):2095-101.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 28  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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