Human IL-12 R beta 1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB839P-100
FAB839P-025
Detection of IL‑12 R beta 1 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (4)
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Human IL-12 R beta 1 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-12 R beta 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-12 R beta 1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 69310
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 1
Cys24-Glu540
Accession # P42701
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-12 R beta 1 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑12 R beta 1 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 5 µg/mL PHA for 48 hours or (B) untreated were stained with Mouse Anti-Human IL-12 R beta 1 PE-conjugated Monoclonal Antibody (Catalog # FAB839P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-12 R beta 1

Interleukin12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta 1 and IL-12 R beta 2. Individually, IL-12 R beta 1 and IL-12 R beta 2 bind IL‑12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I transmembrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 1 cDNA encodes a 662 amino acid (aa) protein with a putative 23 aa signal peptide that is cleaved to generate the mature protein with a 522 aa extracellular domain, a 25 aa transmembrane domain and a 92 aa cytoplasmic region. Expression of IL-12 R beta 1 is detected in activated T cells, NK cells and B cells. The expression of IL‑12 R beta 2 is more restricted and appears to be limited to Th2 cells.

References
  1. Gately, M.K. et al. (1998) Annu. Rev. Immunol. 16:495.
Long Name
Interleukin 12 Receptor beta 1
Entrez Gene IDs
3594 (Human); 16161 (Mouse)
Alternate Names
CD212 antigen; CD212; IL-12 R beta 1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R beta 1; IL-12R subunit beta-1; IL12R; IL12RB; IL12RB1; IL-12Rb1; IL-12R-BETA1; IL-12R-beta-1; interleukin 12 receptor, beta 1; interleukin-12 receptor beta-1 chain; interleukin-12 receptor subunit beta-1; MGC34454

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Citations for Human IL-12 R beta 1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Synoviocytes and skin fibroblasts show opposite effects on IL-23 production and IL-23 receptor expression during cell interactions with immune cells
    Authors: M Noack, P Miossec
    Arthritis Research & Therapy, 2022-09-10;24(1):220.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. IL-21 in the bone marrow microenvironment contributes to IgM secretion and proliferation of malignant cells in Waldenstrom macroglobulinemia.
    Authors: Hodge, Lucy S, Ziesmer, Steve C, Yang, Zhi Zhan, Secreto, Frank J, Gertz, Morie A, Novak, Anne J, Ansell, Stephen
    Blood, 2012-09-13;120(18):3774-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo.
    Authors: Cocco C, Di Carlo E, Zupo S
    Leukemia, 2011-12-23;26(6):1365-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A novel form of complete IL-12/IL-23 receptor beta1 deficiency with cell surface-expressed nonfunctional receptors.
    Authors: Fieschi C, Bosticardo M, de Beaucoudrey L, Boisson-Dupuis S, Feinberg J, Santos OF, Bustamante J, Levy J, Candotti F, Casanova JL
    Blood, 2004-06-03;104(7):2095-101.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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