Human IL-13 R alpha 1 PE-conjugated Antibody

  (2 citations)     
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Citations (2)
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  • Species Reactivity
    Human
  • Specificity
    Detects human IL-13 R alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-13 R alpha 1, recombinant human (rh) IL-13 R alpha 2, rhIL-4 R, rhIL-5 R beta, or rhIL-9 R is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 419718
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 1
    Ala27-Thr343
    Accession # P78552
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of IL‑13 R alpha 1 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with (A) MouseAnti-Human Siglec-3/CD33 APC-conjugated Monoclonal Antibody (Catalog # FAB1137A) and (B) Mouse Anti-Human IL‑13 R alpha 1 PE‑conjugated Monoclonal Antibody (Catalog # FAB1462P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-13 R alpha 1

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

The human IL-13 R alpha 1 was originally cloned based on sequence homology to the mouse IL-13 R alpha 1. The IL-13 R alpha 1 cDNA encodes a 427 amino acid (aa) precursor protein with a putative 21 aa signal peptide, a 324 aa extracellular domain, a 23 aa transmembrane region and a 59 aa cytoplasmic tail. Human and mouse IL-13 R alpha 1 share 76% aa sequence identity. The extracellular domain of IL-13 R alpha 1 is also closely related to that of IL-13 R alpha 2. IL‑13 R alpha 1 has been shown to combine with the IL-4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated.

  • References:
    1. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
    2. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
    3. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
    4. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
    5. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
  • Long Name:
    Interleukin 13 Receptor alpha 1
  • Entrez Gene IDs:
    3597 (Human); 16164 (Mouse)
  • Alternate Names:
    bB128O4.2.1 (interleukin 13 receptor, alpha 1); Cancer/testis antigen 19; CD213a1 antigen; CD213a1; CT19; IL-13 R alpha 1; IL13 receptor alpha-1 chain; IL-13 receptor subunit alpha-1; IL13R alpha 1; IL-13R subunit alpha-1; IL13R; IL13RA; IL-13Ra; IL13RA1; IL-13Ra1; IL-13R-alpha-1; interleukin 13 receptor, alpha 1; interleukin-13 receptor subunit alpha-1; NR4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Tumour-derived PGD2 and NKp30-B7H6 engagement drives an immunosuppressive ILC2-MDSC axis
    Authors: S Trabanelli, MF Chevalier, A Martinez-U, A Gomez-Cade, B Salomé, M Lecciso, V Salvestrin, G Verdeil, J Racle, C Papayannid, H Morita, I Pizzitola, C Grandcléme, P Bohner, E Bruni, M Girotra, R Pallavi, P Falvo, EO Leibundgut, GM Baerlocher, C Carlo-Stel, D Taurino, A Santoro, O Spinelli, A Rambaldi, E Giarin, G Basso, C Tresoldi, F Ciceri, D Gfeller, CA Akdis, L Mazzarella, S Minucci, PG Pelicci, E Marcenaro, ANJ McKenzie, D Vanhecke, G Coukos, D Mavilio, A Curti, L Derré, C Jandus
    Nat Commun, 2017;8(1):593.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Suppression of human glioma xenografts with second-generation IL13R-specific chimeric antigen receptor-modified T cells.
    Authors: Kong S, Sengupta S, Tyler B, Bais A, Ma Q, Doucette S, Zhou J, Sahin A, Carter B, Brem H, Junghans R, Sampath P
    Clin Cancer Res, 2012;18(21):5949-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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Isotype Controls
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Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 24  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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