Human IL-16 Quantikine ELISA Kit

  (11 citations)
(1 Review)
    
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Assay Procedure
Citations (11)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
  • Sensitivity
    13.4 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human IL-16
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC21 , Quantikine Immunoassay Control Group 4 - Please Inquire
Product Summary
The Quantikine Human IL-16 Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IL-16 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-16 and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human IL-16. Results obtained using natural IL-16 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-16.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 205 615 1066 199 588 1135
Standard Deviation 11.4 20.6 32.5 10.7 34.3 63.8
CV% 5.6 3.3 3 5.4 5.8 5.6

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 241 747 1456 230 672 1315
Standard Deviation 12.3 38.4 59.9 27.5 77.1 156
CV% 5.1 5.1 4.1 12 11.5 11.9

Recovery

The recovery of human IL-16 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 94-106
EDTA Plasma (n=6) 105 96-117
Heparin Plasma (n=6) 102 86-112
Serum (n=6) 103 94-113
Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of IL-16 were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 IL-16 [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-16

Interleukin 16 (IL-16), also named lymphocyte chemoattractant factor (LCF), is a 14-17 kDa single chain non-glycosylated polypeptide that was originally identified as a CD8+ T cell-derived chemoattractant for CD4+ cells. The expression of IL-16 precursor mRNA has been detected in various tissues including spleen, thymus, lymph nodes, peripheral leukocytes, bone marrow and cerebellum. In addition to its chemotactic properties, IL-16 has also been shown to suppress HIV-1 replication in vitro.

  • Long Name:
    Interleukin 16
  • Entrez Gene IDs:
    3603 (Human); 16170 (Mouse)
  • Alternate Names:
    FLJ16806; FLJ42735; FLJ44234; HsT19289; IL16; IL-16; interleukin 16 (lymphocyte chemoattractant factor); LCF; LCFprIL-16; lymphocyte chemoattractant factor; neuronal interleukin 16; NIL16; PRIL16; prointerleukin 16; pro-interleukin-16
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Sample Type
  1. The protective effects of magnolol on acute trinitrobenzene sulfonic acid?induced colitis in rats
    Authors: Y Zhang, LT Fu, F Tang
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Type: Serum
  2. Correlation of serum cartilage oligometric matrix protein (COMP) and interleukin-16 (IL-16) levels with disease severity in primary knee osteoarthritis: A pilot study in a Malaysian population
    Authors: E Das Gupta, WR Ng, SF Wong, AK Bhurhanude, SS Yeap
    PLoS ONE, 2017;12(9):e0184802.
    Species: Human
    Sample Type: Serum
  3. In type 1 diabetes immunocompetent cells are defective in IL-16 secretion.
    Authors: Vendrame F, Cataldo D, Ciarlo L
    Scand. J. Immunol., 2012;75(1):127-8.
    Species: Human
    Sample Type: Serum
  4. IL-16 promotes T. whipplei replication by inhibiting phagosome conversion and modulating macrophage activation.
    Authors: Ghigo E, Barry AO, Pretat L, Al Moussawi K, Desnues B, Capo C, Kornfeld H, Mege JL
    PLoS ONE, 2010;5(10):e13561.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. Interleukin-16 in tuberculous and malignant pleural effusions.
    Authors: Qin XJ, Shi HZ, Huang ZX, Kang LF, Mo WN, Wu C
    Eur. Respir. J., 2005;25(4):605-11.
    Species: Human
    Sample Type: Serum
  6. Interleukin-12 and interleukin-16 in periodontal disease.
    Authors: Tsai IS, Tsai CC, Ho YP, Ho KY, Wu YM, Hung CC
    Cytokine, 2005;31(1):34-40.
    Species: Human
    Sample Type: Gingival Crevicular Fluid (GCF)
  7. Interleukin-18 levels correlate with severe ovarian hyperstimulation syndrome.
    Authors: Barak V, Elchalal U, Edelstein M, Kalickman I, Lewin A, Abramov Y
    Fertil. Steril., 2004;82(2):415-20.
    Species: Human
    Sample Type: Plasma
  8. Serum level of interleukin-16 in multiple myeloma patients and its relationship to disease activity.
    Authors: Alexandrakis MG, Passam FH, Kyriakou DS, Christophoridou AV, Perisinakis K, Hatzivasili A, Foudoulakis A, Castanas E
    Am. J. Hematol., 2004;75(2):101-6.
    Species: Human
    Sample Type: Serum
  9. Impact of NNRTI compared to PI-based highly active antiretroviral therapy on CCR5 receptor expression, beta-chemokines and IL-16 secretion in HIV-1 infection.
    Authors: Burton CT, Hardy GA, Sullivan AK, Nelson MR, Gazzard B, Gotch FM, Imami N
    Clin. Exp. Immunol., 2002;130(2):286-92.
    Species: Human
    Sample Type: Plasma
  10. Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
    Authors: Binninger-Schinzel D, Norley S, Adler HS, Oberg HH, Kurth R
    Virology, 2002;294(1):209-21.
    Species: Human
    Sample Type: Cell Culture Supernates
  11. An analysis of acute changes in interleukin-6 levels after treatment of hepatitis C with consensus interferon.
    Authors: Cotler SJ, Reddy KR, McCone J, Wolfe DL, Liu A, Craft TR, Ferris MW, Conrad AJ, Albrecht J, Morrissey M, Ganger DR, Rosenblate H, Blatt LM, Jensen DM, Taylor MW
    J. Interferon Cytokine Res., 2001;21(12):1011-9.
    Species: Human
    Sample Type: Serum
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Average Rating: 5 (Based on 1 review)

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