Human IL-17/IL-17A Alexa Fluor® 700-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human IL-17 in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17 is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 41809
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL-17
    Accession # Q16552
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑17/IL‑17A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Mouse Anti-Human IL‑17/IL‑17A Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # IC317N) and Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # IC0041N). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-17/IL-17A
Interleukin 17, also known as IL-17A and CTLA-8, is a 20-22 kDa, secreted pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield the 132 aa residue mature IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 is also known to form a heterodimer with 20-24 kDa IL-17F. At the amino acid level, hIL-17 shows 62% aa sequence identity with mouse IL-17. The receptor for the IL-17A homodimer and IL-17A:F heterodimer is reported to be a combination of IL-17 RA and IL-17 RC, with a possible contribution by IL-17 RD. The expression of IL-17 is widespread, and found associated with mast cells, LTi cells, B cells, gamma δ T cells, CD4+ Th17 cells, iNKT cells, neutrophils, intestinal Paneth cells, Type I ILCs and CD8+ Tc17 cells. IL-17 exhibits multiple biological activities on a variety of cells, including: the induction of IL-6 and IL-8 production by fibroblasts, the enhancement of surface expression of ICAM-1 on fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation, the preservation of intestinal mucosal integrity via claudin synthesis, and the induction of antimicrobial peptides by epithelium.
  • Long Name:
    Interleukin 17
  • Entrez Gene IDs:
    3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
  • Alternate Names:
    CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 700-conjugated Isotype Control

Ctrl IC0041N
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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