Human/Primate IL-17/IL-17A Antibody

Name: Human/Primate IL-17/IL-17A Antibody
Brand: R&D Systems
SKU: MAB317-500
Rating: 5 (3 reviews)

Catalog #: MAB317
21 Citations 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB317-SP
MAB317-100
MAB317-500

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IL‑17/IL‑17A in Human Crohn's Disease Intestine.

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Product Details

Citations (21)

FAQs

Supplemental Products

Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Primate IL-17/IL-17A Antibody Summary

Species Reactivity

Human, Primate

Specificity

Detects human and primate IL-17 in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17 is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.

Source

Monoclonal Mouse IgG_2B Clone # 41809

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Immunoprecipitation

3 µg/100 µg cell lysate

See below

Human/Primate IL-17 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human/Primate IL‑17/IL‑17A Biotinylated Antibody (Catalog # BAF317)

Standard: Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB)

Neutralization

Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND₅₀) is typically 1-3 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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IL‑17/IL‑17A in Human Crohn's Disease Intestine. IL-17/IL-17A was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Mouse Anti-Human/Primate IL-17/IL-17A Monoclonal Antibody (Catalog # MAB317) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunoprecipitation

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Immunoprecipitation of Human IL‑17. Human IL-17 was immunoprecipitated from 100 µg of human primary differentiated Th17 cell lysate following incubation with 3 µg Mouse Anti-Human/Primate IL-17 Monoclonal Antibody (Catalog # MAB317) or isotype control antibody (Catalog # MAB004) overnight at 4 °C. IL-17-antibody complexes were absorbed using anti-mouse agarose beads. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

Neutralization

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IL‑6 Secretion Induced by IL‑17 and Neutralization by Human IL‑17 Antibody. Recombinant Human IL-17 (Catalog # 317-ILB) stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Human IL-17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-17 Monoclonal Antibody (Catalog # MAB317). The ND₅₀ is typically 1-3 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

IL-17 is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Long Name

Interleukin 17

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Primate IL-17/IL-17A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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Establishment of a humanized animal model of systemic sclerosis in which T helper-17 cells from patients with systemic sclerosis infiltrate and cause fibrosis in the lungs and skin
Authors: MJ Park, Y Park, JW Choi, JA Baek, HY Jeong, HS Na, YM Moon, ML Cho, SH Park
Experimental & Molecular Medicine, 2022;54(9):1577-1585.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation
Authors: J Yan, SP Pandey, BJ Barnes, JR Turner, C Abraham
Cell Rep, 2020;31(13):107820.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Capture
The vitamin D analogue calcipotriol promotes an anti-tumorigenic phenotype of human pancreatic CAFs but reduces T cell mediated immunity
Authors: L Gorchs, S Ahmed, C Mayer, A Knauf, C Fernández, M Svensson, R Heuchel, E Rangelova, P Bergman, H Kaipe
Sci Rep, 2020;10(1):17444.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis
Authors: Y Liao, J Zhao, K Bulek, F Tang, X Chen, G Cai, S Jia, PL Fox, E Huang, TT Pizarro, MF Kalady, MW Jackson, S Bao, GC Sen, GR Stark, CJ Chang, X Li
Nat Commun, 2020;11(1):900.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Fisetin, a 3,7,3',4'-Tetrahydroxyflavone Inhibits the PI3K/Akt/mTOR and MAPK Pathways and Ameliorates Psoriasis Pathology in 2D and 3D Organotypic Human Inflammatory Skin Models
Authors: JC Chamcheu, S Esnault, VM Adhami, AL Noll, S Banang-Mbe, T Roy, SS Singh, S Huang, KG Kousoulas, H Mukhtar
Cells, 2019;8(9):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Serial Monitoring of Immune Markers Being Represented Regulatory T Cell/T Helper 17 Cell Ratio: Indicating Tolerance for Tapering Immunosuppression after Liver Transplantation
Authors: J Jhun, SH Lee, SK Lee, HY Kim, ES Jung, DG Kim, J Choi, SH Bae, SK Yoon, BH Chung, CW Yang, ML Cho, JY Choi
Front Immunol, 2018;9(0):352.
Species: Human
Sample Types: Plasma
Applications: ELISA Capture
Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
PLoS ONE, 2017;12(4):e0175712.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)
IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
Cell. Physiol. Biochem., 2017;42(5):1961-1972.
Species: Human
Sample Types: Serum
Applications: ELISA Development (Capture)
Increase in circulating Th17 cells during anti-TNF therapy is associated with ultrasonographic improvement of synovitis in rheumatoid arthritis
Authors: Dobrina N Hull
Arthritis Res. Ther, 2016;18(1):303.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)
Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage.
Authors: Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S
Eur J Immunol, 2015;46(2):440-5.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
Cytokine, 2013;64(3):660-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development

TH17 cytokines induce human airway smooth muscle cell migration.
Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
Immunol. Lett., 2010;127(2):150-6.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Cytokine-induced CEACAM1 expression on keratinocytes is characteristic for psoriatic skin and contributes to a prolonged lifespan of neutrophils.
Authors: Rahmoun M, Moles JP, Pedretti N, Mathieu M, Fremaux I, Raison-Peyron N, Lecron JC, Yssel H, Pene J
J. Invest. Dermatol., 2009;129(3):671-81.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
J. Immunol., 2009;182(0):3112-20.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

IL-17RA and IL-17RC receptors are essential for IL-17A-induced ELR+ CXC chemokine expression in synoviocytes and are overexpressed in rheumatoid blood.
Authors: Zrioual S, Toh ML, Tournadre A, Zhou Y, Cazalis MA, Pachot A, Miossec V, Miossec P
J. Immunol., 2008;180(1):655-63.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
J. Immunol., 2008;181(6):4299-310.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007;329(1):214-8.
Species: Human
Sample Types: Serum
Applications: Luminex Development

Ultrasensitive flow-based immunoassays using single-molecule counting.
Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clin. Chem., 2007;53(11):1990-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development

IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
J. Immunol., 2005;175(9):6177-89.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
J. Immunol., 2004;173(2):1463-76.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry