Human/Primate IL-17/IL-17A Antibody

(13 citations)   
  • Species Reactivity
    Human, Primate
  • Specificity
    Detects human and primate IL-17 in direct ELISAs. In direct ELISAs, approximately 12% cross-reactivity with recombinant canine IL-17 is observed and 25%-50% reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 41809
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human IL-17
    Ile20-Ala155
    Accession # Q16552
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunoprecipitation
    3 µg/100 µg cell lysate
    See below
    • Human/Primate IL-17 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human/Primate IL‑17/IL‑17A Antibody (Catalog # MAB317)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human/Primate IL‑17/IL‑17A Biotinylated Antibody (Catalog # BAF317)
  • ELISA Standard
     
    Recombinant Human IL-17A Protein, CF (Catalog # 317-ILB)
  • Neutralization
    Measured by its ability to neutralize IL‑17-induced IL‑6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 15 ng/mL Recombinant Human IL‑17.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunoprecipitation
Immunoprecipitation of Human IL‑17.

Human IL-17 was immunoprecipitated from 100 μg of human primary differentiated Th17 cell lysate following incubation with 3 µg Mouse Anti-Human/Primate IL-17 Monoclonal Antibody (Catalog # MAB317) or isotype control antibody (Catalog # MAB004) overnight at 4 °C. IL-17-antibody complexes were absorbed using anti-mouse agarose beads. Immunoprecipitated IL-17 was detected by Western blot using 1 µg/mL Goat Anti-Human IL-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-317-NA). View our recommended buffer recipes for immunoprecipitation.

IL‑6 Secretion Induced by
IL‑17 and Neutralization by Human IL‑17 Antibody.

Recombinant Human IL‑17 (Catalog # 317-ILB) stimulates IL‑6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL‑6 Quantikine ELISA Kit (Catalog # M6000B). IL‑6 secretion elicited by Recombinant Human IL‑17 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL‑17 Monoclonal Antibody (Catalog # MAB317). The ND50 is typically 1-3 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17/IL-17A
IL-17 is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.
  • Long Name:
    Interleukin 17
  • Entrez Gene IDs:
    3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
  • Alternate Names:
    CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017;42(5):1961-1972.
    Species: Human
    Sample Type: Serum
    Application: ELISA Capture
  2. Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
    Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
    PLoS ONE, 2017;12(4):e0175712.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Capture
  3. Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage.
    Authors: Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S
    Eur J Immunol, 2015;46(2):440-5.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
    Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
    Cytokine, 2013;64(3):660-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  5. TH17 cytokines induce human airway smooth muscle cell migration.
    Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
    J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
    Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
    Immunol. Lett., 2010;127(2):150-6.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  7. Cytokine-induced CEACAM1 expression on keratinocytes is characteristic for psoriatic skin and contributes to a prolonged lifespan of neutrophils.
    Authors: Rahmoun M, Moles JP, Pedretti N, Mathieu M, Fremaux I, Raison-Peyron N, Lecron JC, Yssel H, Pene J
    J. Invest. Dermatol., 2009;129(3):671-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  8. Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes.
    Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P
    J. Immunol., 2009;182(0):3112-20.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities.
    Authors: Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL
    J. Immunol., 2008;181(6):4299-310.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  10. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Type: Serum
    Application: Luminex Development
  11. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007;53(11):1990-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  12. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  13. IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
    Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
    J. Immunol., 2004;173(2):1463-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 13 Citations
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