Human CD4 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3791P-100
FAB3791P-025
Human CD4 PE-conjugated Antibody in Flow Cytometry
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Product Details
Citations (6)
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Human CD4 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD4 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 11830
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant Human CD4.
Extracellular Domain
Accession # P01730
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD4 in Human Blood Lymphocytes by Flow Cytometry. View Larger

Detection of CD4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) or (B) Mouse IgG2A Phycoerythrin Isotype Control (Catalog # IC003P) and Mouse Anti-Human CD3 epsilon CFS‑conjugated Monoclonal Antibody (Catalog # FAB100F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD4

CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Entrez Gene IDs
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline)
Alternate Names
CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Product Datasheets

Citations for Human CD4 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Phosphatase inhibitor PPP1R11 modulates resistance of human T cells toward Treg-mediated suppression of cytokine expression
    Authors: RN Joshi, SJ Fernandes, MM Shang, NA Kiani, D Gomez-Cabr, J Tegnér, A Schmidt
    J. Leukoc. Biol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Therapeutic immune monitoring of CD4(+)CD25(+) T cells in chronic myeloid leukemia patients treated with tyrosine kinase inhibitors
    Authors: Z Lu, N Xu, X Zhou, G Gao, L Li, J Huang, Y Li, Q Lu, B He, C Pan, X Liu
    Oncol Lett, 2017;14(2):1363-1372.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Nuclear trafficking of the HIV-1 pre-integration complex depends on the ADAM10 intracellular domain.
    Authors: Endsley M, Somasunderam A, Li G, Oezguen N, Thiviyanathan V, Murray J, Rubin D, Hodge T, O'Brien W, Lewis B, Ferguson M
    Virology, 2014;454(0):60-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation.
    Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A
    2011;6(5):e19988.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Chronically inflamed human tissues are infiltrated by highly differentiated Th17 lymphocytes.
    Authors: Pene J, Chevalier S, Preisser L, Venereau E, Guilleux MH, Ghannam S, Moles JP, Danger Y, Ravon E, Lesaux S, Yssel H, Gascan H
    J. Immunol., 2008;180(0):7423.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Chemokine receptor expression on allergen-specific T cells in asthma and allergic bronchopulmonary aspergillosis.
    Authors: Garcia G, Humbert M, Capel F, Rimaniol AC, Escourrou P, Emilie D, Godot V
    Allergy, 2007;62(2):170-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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