Human IL-17 RE APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8358A-025
Detection  of IL-17 RE in HEK293 Human Cell Line Transfected with Human IL-17 RE and eGFP  by Flow Cytometry.
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Human IL-17 RE APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17 RE in direct ELISAs. By flow cytometry, this antibody is specific for IL-17 RE and does not bind to cells transfected with IL-17 RA, IL-17 RB, or IL-17 RC.
Source
Monoclonal Mouse IgG1 Clone # 934832
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human IL-17 RE
Met1-His454
Accession # Q8NFR9
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of IL-17 RE in HEK293 Human Cell Line Transfected with Human IL-17 RE and eGFP  by Flow Cytometry. View Larger

Detection of IL-17 RE in HEK293 Human Cell Line Transfected with Human IL-17 RE and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human IL-17 RE and eGFP was stained with (A) Mouse Anti-Human IL-17 RE APC‑conjugated Monoclonal Antibody (Catalog # FAB8358A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17 RE

Interleukin-17 Receptor E (IL-17 RE) is an approximately 70 kDa (predicted) transmembrane protein in the family of IL-17 receptors. IL-17 RE is required for mediating the pro-inflammatory and homeostatic actions of IL-17C in the skin and mucosa (1, 2). Mature human IL-17 RE consists of a 431 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 192 aa cytoplasmic domain with one SEFIR/TIR motif (3). Within aa 115-454, human IL-17 RE shares 79% aa sequence identity with mouse and rat IL-17 RE. Alternative splicing of human IL-17 RE generates additional isoforms with a 116 aa N-terminal deletion and/or a substitution with truncation in the ECD following aa 268 or aa 433. IL-17 RE is expressed on keratinocytes, mucosal epithelial cells, Th17 cells, and gamma /δ T cells (4, 5). It associates with the widely expressed IL-17 RA to form a heterodimeric receptor for IL-17C (4-6). IL-17C binds to IL-17 RE with high affinity and to IL-17 RA with low affinity (4, 5). IL-17C expression is induced by inflammatory stimuli in colon and airway epithelial cells, keratinocytes, CD4+ T cells, macrophages, and dendritic cells (4, 6, 7-9). It is up-regulated in various chronic inflammatory diseases including psoriasis, cystic fibrosis, and chronic obstructive pulmonary disease (COPD) (7, 8, 10). IL-17 RE is reciprocally down-regulated in psoriatic lesions (10). The interaction of IL-17C with IL-17 RE promotes mucosal immunity through the induction of anti-bacterial peptides and pro-inflammatory cytokines and chemokines (4, 6, 8, 9). IL-17C action supports the integrity of the colon epithelium following infection induced damage (4, 6, 11) but also contributes to psoriatic skin thickening and the progression of arthritis (4, 8, 9). IL-17C is additionally up-regulated in Th17 cell dependent autoimmunity (5). In this setting, it exacerbates disease severity by inducing Th17 cell production of IL-17A, IL-17F, IL-22, CCR6, and CCL20 (5). The up-regulation of IL-17 RE in hepatocellular carcinoma is associated with poor prognosis (12).

References
  1. Pappu, R. et al. (2012) Trends Immunol. 33:343.
  2. Rubino, S.J. et al. (2012) Trends Immunol. 33:112.
  3. Li, T.S. et al. (2006) Cell. Signal. 18:1287.
  4. Ramirez-Carrozzi, V. et al. (2011) Nat. Immunol. 12:1159.
  5. Chang, S.H. et al. (2011) Immunity 35:611.
  6. Song, X. et al. (2011) Nat. Immunol. 12:1151.
  7. Pfeifer, P. et al. (2013) Am. J. Respir. Cell Mol. Biol. 48:415.
  8. Johnston, A. et al. (2013) J. Immunol. 190:2252.
  9. Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128.
  10. Johansen, C. et al. (2009) Br. J. Dermatol. 160:319.
  11. Reynolds, J.M. et al. (2012) J. Immunol. 189:4226.
  12. Liao, R. et al. (2013) J. Exp. Clin. Cancer Res. 32:3.
Long Name
Interleukin 17 Receptor E
Entrez Gene IDs
132014 (Human); 57890 (Mouse); 362417 (Rat)
Alternate Names
IL-17 RE; IL-17 receptor E; IL17RE; IL-17RE; Il25r; interleukin 17 receptor E

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