Human IL-17 RE APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human IL-17 RE in direct ELISAs. By flow cytometry, this antibody is specific for IL-17 RE and does not bind to cells transfected with IL-17 RA, IL-17 RB, or IL-17 RC.
  • Source
    Monoclonal Mouse IgG1 Clone # 934832
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human IL-17 RE
    Accession # Q8NFR9
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL-17 RE in HEK293 Human Cell Line Transfected with Human IL-17 RE and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human IL-17 RE and eGFP was stained with (A) Mouse Anti-Human IL-17 RE APC‑conjugated Monoclonal Antibody (Catalog # FAB8358A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-17 RE
Interleukin-17 Receptor E (IL-17 RE) is an approximately 70 kDa (predicted) transmembrane protein in the family of IL-17 receptors. IL-17 RE is required for mediating the pro-inflammatory and homeostatic actions of IL-17C in the skin and mucosa (1, 2). Mature human IL-17 RE consists of a 431 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 192 aa cytoplasmic domain with one SEFIR/TIR motif (3). Within aa 115-454, human IL-17 RE shares 79% aa sequence identity with mouse and rat IL-17 RE. Alternative splicing of human IL-17 RE generates additional isoforms with a 116 aa N-terminal deletion and/or a substitution with truncation in the ECD following aa 268 or aa 433. IL-17 RE is expressed on keratinocytes, mucosal epithelial cells, Th17 cells, and gamma /δ T cells (4, 5). It associates with the widely expressed IL-17 RA to form a heterodimeric receptor for IL-17C (4-6). IL-17C binds to IL-17 RE with high affinity and to IL-17 RA with low affinity (4, 5). IL-17C expression is induced by inflammatory stimuli in colon and airway epithelial cells, keratinocytes, CD4+ T cells, macrophages, and dendritic cells (4, 6, 7-9). It is up-regulated in various chronic inflammatory diseases including psoriasis, cystic fibrosis, and chronic obstructive pulmonary disease (COPD) (7, 8, 10). IL-17 RE is reciprocally down-regulated in psoriatic lesions (10). The interaction of IL-17C with IL-17 RE promotes mucosal immunity through the induction of anti-bacterial peptides and pro-inflammatory cytokines and chemokines (4, 6, 8, 9). IL-17C action supports the integrity of the colon epithelium following infection induced damage (4, 6, 11) but also contributes to psoriatic skin thickening and the progression of arthritis (4, 8, 9). IL-17C is additionally up-regulated in Th17 cell dependent autoimmunity (5). In this setting, it exacerbates disease severity by inducing Th17 cell production of IL-17A, IL-17F, IL-22, CCR6, and CCL20 (5). The up-regulation of IL-17 RE in hepatocellular carcinoma is associated with poor prognosis (12).
  • References:
    1. Pappu, R. et al. (2012) Trends Immunol. 33:343.
    2. Rubino, S.J. et al. (2012) Trends Immunol. 33:112.
    3. Li, T.S. et al. (2006) Cell. Signal. 18:1287.
    4. Ramirez-Carrozzi, V. et al. (2011) Nat. Immunol. 12:1159.
    5. Chang, S.H. et al. (2011) Immunity 35:611.
    6. Song, X. et al. (2011) Nat. Immunol. 12:1151.
    7. Pfeifer, P. et al. (2013) Am. J. Respir. Cell Mol. Biol. 48:415.
    8. Johnston, A. et al. (2013) J. Immunol. 190:2252.
    9. Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128.
    10. Johansen, C. et al. (2009) Br. J. Dermatol. 160:319.
    11. Reynolds, J.M. et al. (2012) J. Immunol. 189:4226.
    12. Liao, R. et al. (2013) J. Exp. Clin. Cancer Res. 32:3.
  • Long Name:
    Interleukin 17 Receptor E
  • Entrez Gene IDs:
    132014 (Human); 57890 (Mouse); 362417 (Rat)
  • Alternate Names:
    IL-17 receptor E; IL17RE; IL-17RE; Il25r; interleukin 17 receptor E
Related Research Areas
Isotype Controls
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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