Human IL-17RC APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB22691A
Detection of IL‑17RC in K562 Human Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Human IL-17RC APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17RC in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑17R, rhIL-17BR, rhIL-17RD, or recombinant mouse IL-17RC is observed.
Source
Monoclonal Mouse IgG2B Clone # 309822
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑17RC
Leu21-Ala454
Accession # NP_116121
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
K562 human chronic myelogenous leukemia cell line and PC‑3 human prostate cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-17 RC antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑17RC in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human IL-17RC APC-conjugated Monoclonal Antibody (Catalog # FAB22691A, filled histogram) or isotype control antibody (IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of IL-17RC in PC-3 cells by Flow Cytometry. PC-3 cells were stained with Mouse Anti-Human IL-17RC APC‑conjugated Monoclonal Antibody (Catalog # FAB22691A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-17RC

IL-17 receptor C (IL-17RC; also known as IL-17RL and IL-17 Rhom) is an 85‑110 kDa member of the IL-17 receptor family. This is one of five families that currently comprise the cytokine receptor superfamily (1, 2, 3, 4, 5, 6). At this time, there are five members within the IL-17 receptor family, and these are termed IL-17RA, B, C, D and E. Not all receptors appear to bind known members of the IL-17 cytokine family. To date, IL-17RA is reported to bind IL-17(A), while IL-17RB is reported to bind IL-17B and IL-17E (2, 4). Human IL-17RC is a type I transmembrane glycoprotein that is expressed on a variety of nonhematopoietic cell types. These include endothelial cells (6, 7), chondrocytes and osteoblasts (8), breast and prostatic epithelium (6), and fibroblasts, plus renal tubular epithelium and skeletal muscle cells (8, 9). Full-length IL-17RC is synthesized as a 791 amino acid (aa) precursor (10, 11). It contains a 20 aa signal sequence, a 518 aa extracellular domain (ECD) (aa 21‑538), a 21 aa transmembrane segment, and a 232 aa cytoplasmic region. Although IL-17RA has two fibrinogen-like regions in its ECD that contribute to its function, no such architecture has been identified in the ECD of IL-17RC (12). Based on studies looking at exon deletions, a key ligand-binding site would appear to exist over aa 425‑441 (13). The gene for human IL-17RC contains 19 exons. It is estimated that there are over 90 alternative splice forms, with transmembrane-containing isoforms predominating (6, 14). The full-length isoform is estimated to occur approximately 10% of the time, while the three most common isoforms, as a group, occur about 50% of the time. Based on limited information, alternative splicing appears to regulate ligand specificity (13). R&D Systems IL-17RC corresponds to IL-17RC isoform # 3, which shows deletions of aa 36‑106 and 264‑278 relative to the full-length form (10). Over the ECD, IL-17RC isoform #3 is 68% aa identical to mouse IL-17RC ECD. IL-17RC is the cognate receptor for IL-17F, and binds IL-17A with similar affinity (13). With IL-17RA, it forms a definitive receptor for both IL-17A and IL-17F. The stoichiometry is unclear; it may form a heterodimer with IL-17RA, or a heterotrimer with a preexisting IL-17RA homodimer (4, 9, 13, 15). The heteromeric nature of the receptor may be important given that the predominant form of the IL-17 cytokine is now considered to be an IL-17A:IL-17F heterodimer (4).

References
  1. Gaffen, S.L. et al. (2006) Vitam. Horm. 74:255.
  2. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:821.
  3. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
  4. Shen, F. and S.L. Gaffen (2008) Cytokine 41:92.
  5. You, Z. et al. (2006) Cancer Res. 66:175.
  6. You, Z. et al. (2007) Neoplasia 9:464.
  7. Gerritsen, M.E. et al. (2003) Br. J. Pharmacol. 140:595.
  8. Kokubu, T. et. Al. (2008) J. Hictochem. Cytochem. 56:89.
  9. Toy, D. et al. (2006) J. Immunol. 177:36.
  10. GenBank Accession # Q96F46.
  11. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  12. Kramer, J.M. et al. (2007) J. Immunol. 179:6379.
  13. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
  14. Haudenschild, D.R. et al. (2006) Prostate 66:1268.
  15. Kramer, J.M. et al. (2006) J. Immunol. 176:711.
Long Name
Interleukin 17 Receptor C, Transcript Variant 3
Entrez Gene IDs
84818 (Human); 171095 (Mouse)
Alternate Names
FLJ95963; FLJ96005; IL-17 RC; IL-17 receptor C; IL-17 RL; IL17F receptor; IL17RC; IL-17RC; IL17Rhom; IL17RL; IL-17RL; IL17-RL; interleukin 17 receptor C; interleukin-17 receptor C; Interleukin-17 receptor homolog; Interleukin-17 receptor-like protein; MGC10763; zcytoR14

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Citations for Human IL-17RC APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Overexpression of IL-17RC associated with ocular sarcoidosis.
    Authors: Wu W, Jin M, Wang Y, Liu B, Shen D, Chen P, Hannes S, Li Z, Hirani S, Jawad S, Sen H, Chan C, Nussenblatt R, Wei L
    J Transl Med, 2014-05-31;12(0):152.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  2. TH17 cytokines induce human airway smooth muscle cell migration.
    Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
    J. Allergy Clin. Immunol., 2011-02-22;127(4):1046-53.e1-2.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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