Human IL-18 R beta /IL-1 R7 Fluorescein-conjugated Antibody
Human IL-18 R beta /IL-1 R7 Fluorescein-conjugated Antibody Summary
Accession # O95256
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑18 R beta /IL‑1 R7 in KG‑1 Human Cell Line by Flow Cytometry. KG-1 human acute myelogenous leukemia cell line was stained with Mouse Anti-Human IL-18 R beta /IL-1 R7 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB118F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-18 R beta/IL-1 R7
IL-18, originally described as an Interferon-gamma Inducing Factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta, also known as IL-1 R7 and AcPL, subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.
- Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
- Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.
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