Human IL-18 R beta /IL-1 R7 PE-conjugated Antibody

(3 citations)   
  • Species Reactivity
  • Specificity
    Detects human IL‑18 R beta /IL‑1 R7 in direct ELISAs and Western blots. In direct ELISAs, 15% cross-reactivity with recombinant human (rh) IL‑1 RII is observed and 2% cross-reactivity with rhIL-1 R9 is observed. Also in direct ELISAs, no cross-reactivity  with rhIL-1 RI, rhIL‑1 RacP/IL‑1 R3, rhIL-1 R4, rhIL-18 R alpha /IL-1 R5, rhIL-1 R6, rhIL-1 R8, or recombinant mouse (rm) IL-18 R beta /IL-1 R7 is observed. In Western blots, approximately 100% cross-reactivity with rmIL-18 R beta is observed and no cross-reactivity with rhIL-18 R alpha /IL-1 R5, rhIL‑1 RacP/IL‑1 R3, rhIL-1 RI, rhIL-1 RII, or rhIL-1 Rrp2/IL-1 R6 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 132029
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL‑18 R beta /IL‑1 R7
    Accession # O95256
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑18 R beta /IL‑1 R7 in KG‑1 Human Cell Line by Flow Cytometry. KG‑1 human acute myelogenous leukemia cell line was stained with Mouse Anti-Human IL‑18 R beta /IL‑1 R7 PE-conjugated Mono­clonal Antibody (Catalog # FAB118P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-18 R beta/IL-1 R7

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL‑18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL‑18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

  • References:
    1. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
    2. Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.
  • Long Name:
    Interleukin 18 Receptor beta
  • Entrez Gene IDs:
    8807 (Human); 16174 (Mouse)
  • Alternate Names:
    Accessory protein-like; AcPL; CD218 antigen-like family member B; CD218b antigen; CD218b; CDw218b; IL-1 R7; IL-18 receptor accessory protein; IL-18 receptor beta; IL18R beta; IL-18RAcP; IL18RAP; IL18RB; IL-18Rb; IL-18Rbeta; IL-18R-beta; IL-1R accessory protein-like; IL1R7; IL-1R7; IL-1R-7; IL-1RAcPL; interleukin 18 receptor accessory protein; Interleukin-1 receptor 7; interleukin-18 receptor accessory protein; Interleukin-18 receptor accessory protein-like; Interleukin-18 receptor beta; MGC120589; MGC120590
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
    Authors: Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N
    J Immunol, 2015;195(5):2141-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Increased expression of Interleukin-18 receptor in blood cells of subjects with Mild Cognitive Impairment and Alzheimer's disease.
    Cytokine, 2013;61(2):360-3.
    Species: Human
    Sample Type: whole cells
    Application: Flow
  3. Defective phosphorylation of interleukin-18 receptor beta causes impaired natural killer cell function in systemic-onset juvenile idiopathic arthritis.
    Authors: De Jager W, Vastert SJ, Beekman JM, Wulffraat NM, Kuis W, Coffer PJ, Prakken BJ
    Arthritis Rheum., 2009;60(9):2782-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 12  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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