Human IL-1 alpha /IL-1F1 (Research Grade Bermekimab Biosimilar) Antibody

Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB10568-100
MAB10568-SP
Detection of IL‑1 alpha /IL‑1F1 (Bermekimab) in LPS-treated Human PBMCs by Flow Cytometry.
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Human IL-1 alpha /IL-1F1 (Research Grade Bermekimab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑1 alpha /IL‑1F1 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Bermekimab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu146
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human IL‑1 alpha /IL‑1F1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
LPS-treated Human PBMC

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry View Larger

Detection of IL‑1 alpha /IL‑1F1 (Bermekimab) in LPS-treated Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were (A) treated overnight with 1 µg/mL LPS or (B) untreated, then stained with Human IL-1a/IL-1F1 (Bermekimab) Monoclonal Antibody (Catalog # MAB10568, filled histogram) or unstained (open histogram), followed by Anti-Human IgG Allophycoerythrin-conjugated Secondary Antibody (F0135). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

Research grade Bermekimab is a biosimilar that binds and blocks binds to IL-1 alpha/IL-1F1. This product can be used in flow cytometry or various assay formats to block or measure IL-1 alpha/IL-1F1

Long Name
Interleukin 1 alpha
Entrez Gene IDs
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine); 101926546 (Cynomolgus Monkey)
Alternate Names
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

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