Human IL-20R alpha Antibody

Catalog # Availability Size / Price Qty
MAB11762
MAB11762-SP
Detection of IL‑20 R alpha in T47D Cell Line by Flow Cytometry.
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Citations (1)
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Human IL-20R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-20 R alpha in direct ELISAs and Western blots. In Western blots, approximately 5‑15% cross‑reactivity with recombinant human (rh) IFN-gamma R2, rhIL-10 R, rhIL-10 R beta, rhIL-20 R beta, and rhIL-22 BP is observed and no cross-reactivity with rhIFN-gamma RI, recombinant mouse IL‑20 R alpha, or rhIL‑22 R is observed.
Source
Monoclonal Mouse IgG1 Clone # 173714
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-20 R alpha
Val30-Lys250
Accession # Q9UHF4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑20 R alpha Fc Chimera (Catalog # 1176-IR)
Flow Cytometry
0.25 µg/106 cells
Human IL‑20 R alpha transfected Baf/3 cells and T47D human breast carcinoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of IL‑20 R alpha in T47D Cell Line by Flow Cytometry. T47D human breast duct carcinoma cell line was stained with Mouse Anti-Human IL-20 Ra Monoclonal Antibody (Catalog # MAB11762, filled histogram) or isotype control antibody (MAB002, open histogram) followed by Anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). Staining was performed using our View our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-20R alpha

IL-20 receptor alpha (IL-20 R alpha ), also named IL-20 R1, CRF2-8, and ZCYTOR7, belongs to the class II cytokine receptor family, which includes 12 members. These receptors are characterized by the patterns of conserved amino acid (aa) residues in their extracellular domains, which are composed of tandem fibronectin type III domains (1). Class II cytokine receptors form heterodimeric signaling receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1).

The gene for human IL-20 R alpha is mapped to chromosome 6 and encodes a 553 aa glycoprotein with a 29 aa signal peptide, a 221 aa extracellular domain, a 24 aa transmembrane region and a 279 aa intracellular domain (2). IL-20 R alpha is widely expressed and is detected at high levels in multiple tissues including skin, testis, heart, placenta, salivary gland and prostate gland (1). The expression of IL-20 R alpha, together with that of IL-20 R beta, is upregulated in psoriatic skin lesions on keratinocytes, immune cells, and endothelial cells (1, 2).

IL-20 R alpha heterodimerizes with IL-20 R beta to form the functional receptor that mediates IL-19, IL-20 and IL-24 signals (3, 4). IL-20 R alpha also heterodimerizes with IL-10 R beta to form the functional receptor complex for IL-26 (5). Binding of these IL-10 family class II cytokines to their functional receptors induces activation of the JAK-STAT signal transduction pathway. At low ligand concentrations, STAT3 has been shown to be the predominant STAT proteins activated through either complexes (3‑5).

References
  1. Kotenko, S.V. (2003) Cytokine & Growth Factor Reviews 13:223.
  2. Xie, M.H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Dumoutier, L. et al. (2001) J. Immunol. 167:3534.
  4. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517s.
  5. Sheikh. F. et al. (2004) J. Immunol. 172:2006.
Long Name
Interleukin 20 Receptor alpha
Entrez Gene IDs
53832 (Human); 237313 (Mouse)
Alternate Names
class II cytokine receptor ZCYTOR7; CRF2-8; Cytokine receptor class-II member 8; Cytokine receptor family 2 member 8; FLJ40993; IL-20 R alpha; IL20R alpha; IL-20R alpha; IL-20R1; IL-20R1IL-20 receptor subunit alpha; IL20RA; IL-20Ra; IL-20R-alpha; interleukin 20 receptor, alpha; interleukin-20 receptor I; interleukin-20 receptor subunit alpha; zcytor7

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Citation for Human IL-20R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-20, an anti-angiogenic cytokine that inhibits COX-2 expression.
    Authors: Heuzé-Vourc'h N, Liu M, Dalwadi H, Baratelli FE, Zhu L, Goodglick L, Pold M, Sharma S, Ramirez RD, Shay JW, Minna JD, Strieter RM, Dubinett SM
    Biochem. Biophys. Res. Commun., 2005;333(2):470-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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