Human IL-22 R alpha 1 PE-conjugated Antibody

(2 citations)   
  • Species Reactivity
  • Specificity
    Detects human IL‑22 R alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) IL-10, rhIL-22BP or rhIL-20 R alpha is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 305405
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha 1
    Accession # Q8N6P7
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL-22 R alpha 1 in COLO 205 Human Cell Line by Flow Cytometry. COLO 205 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human IL-22 R alpha 1 PE-conjugated Monoclonal Anti­body (Catalog # FAB2770P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-22 R alpha 1

IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta  is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22%‑25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.

  • References:
    1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
    2. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
    3. Boniface, K. et al. (2005) J. Immunol. 174:3695.
    4. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
    5. Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
    6. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
    7. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
    8. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
    9. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
    10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
    11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
    12. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
    13. Wolk, K. et al. (2004) Immunity 21:241.
    14. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
  • Long Name:
    Interleukin 22 Receptor
  • Entrez Gene IDs:
    58985 (Human); 230828 (Mouse)
  • Alternate Names:
    CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 receptor subunit alpha-1; IL22R alpha 1; IL22R; IL22R1; IL22RA1; IL-22Ra1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. TH17 cytokines induce human airway smooth muscle cell migration.
    Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
    J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression.
    Authors: Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K
    Eur. J. Immunol., 2009;39(10):2779-88.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
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Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 28  
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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