Human IL-22 R alpha 1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2770A
Detection of IL‑22 R alpha 1 in COLO 205 Human Cell Line by Flow Cytometry.
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Human IL-22 R alpha 1 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑22 R alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) IL-10, rhIL-22BP or rhIL-20 R alpha is observed.
Source
Monoclonal Mouse IgG1 Clone # 305405
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha 1
Pro18-Thr228
Accession # Q8N6P7
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IL‑22 R alpha 1 in COLO 205 Human Cell Line by Flow Cytometry. View Larger

Detection of IL‑22 R alpha 1 in COLO 205 Human Cell Line by Flow Cytometry. COLO 205 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human IL‑22 R alpha 1 APC‑conjugated Monoclonal Antibody (Catalog # FAB2770A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-22 R alpha 1

IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL‑22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta  is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding IL‑22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22%‑25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.

References
  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Boniface, K. et al. (2005) J. Immunol. 174:3695.
  4. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  5. Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
  6. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
  7. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
  8. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
  9. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
  10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
  11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
  12. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
  13. Wolk, K. et al. (2004) Immunity 21:241.
  14. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
Long Name
Interleukin 22 Receptor
Entrez Gene IDs
58985 (Human); 230828 (Mouse)
Alternate Names
CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 R alpha 1; IL-22 receptor subunit alpha-1; IL22R alpha 1; IL22R; IL22R1; IL22RA1; IL-22Ra1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; ZcytoR11

Product Datasheets

Citations for Human IL-22 R alpha 1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. T cell-derived IL-22 amplifies IL-1beta-driven inflammation in human adipose tissue: relevance to obesity and type 2 diabetes.
    Authors: Dalmas E, Venteclef N, Caer C, Poitou C, Cremer I, Aron-Wisnewsky J, Lacroix-Desmazes S, Bayry J, Kaveri S, Clement K, Andre S, Guerre-Millo M
    Diabetes, 2014;63(6):1966-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. A novel role for IL-22R1 as a driver of inflammation.
    Authors: Savan R, McFarland AP, Reynolds DA
    Blood, 2011;117(2):575-84.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  3. Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells.
    Authors: Maarof G, Bouchet-Delbos L, Gary-Gouy H, Durand-Gasselin I, Krzysiek R, Dalloul A
    Blood, 2010;115(9):1718-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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