Human IL-22 R alpha 1 Antibody

Catalog # Availability Size / Price Qty
MAB2770
MAB2770-SP
Detection of IL‑22 R alpha 1 in COLO 205 Human Cell Line by Flow Cytometry.
2 Images
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (2)

Human IL-22 R alpha 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑22 R alpha 1 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) IL-10, rhIL-22BP or rhIL-20 R alpha is observed.
Source
Monoclonal Mouse IgG1 Clone # 305405
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha 1
Pro18-Thr228
Accession # Q8N6P7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of IL-22 Ra1 antibody in COLO 205 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑22 R alpha 1 in COLO 205 Human Cell Line by Flow Cytometry. COLO 205 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human IL-22 Ra1 Monoclonal Antibody (Catalog # MAB2770, filled histogram) or isotype control antibody (Catalog # MAB002, open histo-gram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry IL-22 Ra1 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC). View Larger

IL‑22 R alpha 1 in HT‑29 Human Cell Line. IL-22 Ra1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human IL-22 Ra1 Monoclonal Antibody (Catalog # MAB2770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-22 R alpha 1

IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL‑22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta  is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding IL‑22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22%‑25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.

References
  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Boniface, K. et al. (2005) J. Immunol. 174:3695.
  4. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  5. Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
  6. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
  7. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
  8. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
  9. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
  10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
  11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
  12. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
  13. Wolk, K. et al. (2004) Immunity 21:241.
  14. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
Long Name
Interleukin 22 Receptor
Entrez Gene IDs
58985 (Human); 230828 (Mouse)
Alternate Names
CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 R alpha 1; IL-22 receptor subunit alpha-1; IL22R alpha 1; IL22R; IL22R1; IL22RA1; IL-22Ra1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11

Product Datasheets

Citations for Human IL-22 R alpha 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Filter by:

  1. Expression of IL-20 Receptor Subunit beta Is Linked to EAE Neuropathology and CNS Neuroinflammation
    Authors: JR Dayton, Y Yuan, LP Pacumio, BG Dorflinger, SC Yoo, MJ Olson, SI Hernández-, MM McMahon, L Cruz-Oreng
    Frontiers in Cellular Neuroscience, 2021;15(0):683687.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection
    Authors: P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader
    Mucosal Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Anti-IL-20 monoclonal antibody inhibited tumor growth in hepatocellular carcinoma
    Authors: YS Chiu, CH Hsing, CF Li, CY Lee, YH Hsu, MS Chang
    Sci Rep, 2017;7(1):17609.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Interleukin-22 drives the proliferation, migration and osteogenic differentiation of mesenchymal stem cells: a novel cytokine that could contribute to new bone formation in spondyloarthropathies
    Authors: Ahmed A El-Zayadi
    Rheumatology (Oxford), 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes.
    Authors: Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier R
    J Neuroinflammation, 2015;12(0):119.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. The crosstalk between IL-22 signaling and miR-197 in human keratinocytes.
    Authors: Lerman, Galya, Sharon, Moran, Leibowitz-Amit, Raya, Sidi, Yechezke, Avni, Dror
    PLoS ONE, 2014;9(9):e107467.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  7. Interleukin-22 suppresses the growth of A498 renal cell carcinoma cells via regulation of STAT1 pathway.
    Authors: Zhang F, Shang D, Zhang Y, Tian Y
    PLoS ONE, 2011;6(5):e20382.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. TH17 cytokines induce human airway smooth muscle cell migration.
    Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
    J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Novel mechanism of MDA-7/IL-24 cancer-specific apoptosis through SARI induction.
    Authors: Dash R, Bhoopathi P, Das S, Sarkar S, Emdad L, Dasgupta S, Sarkar D, Fisher P
    Cancer Res, 0;74(2):563-74.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human IL-22 R alpha 1 Antibody

Average Rating: 4 (Based on 2 Reviews)

5 Star
50%
4 Star
0%
3 Star
50%
2 Star
0%
1 Star
0%

Have you used Human IL-22 R alpha 1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human IL-22 R alpha 1 Antibody
By Anonymous on 11/03/2021
Application: ICC/IF Sample Tested: Dermal cells,Epidermal cells Species: Human

Human IL-22 R alpha 1 Antibody
By Anonymous on 06/09/2020
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody in an in-house ELISA along with pAb (AF2770) and protein standard (2770-LR) to quantify IL-22R alpha1 in human serum and plasma. This combination could not detect IL-22R alpha1 in our samples but generated a good standard curve.