Human IL-22 R alpha 1 Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL‑22 R alpha 1 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) IL-10, rhIL-22BP or rhIL-20 R alpha is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 305405
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha 1
    Pro18-Thr228
    Accession # Q8N6P7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑22 R alpha 1 in COLO 205 Human Cell Line by Flow Cytometry. COLO 205 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB2770, filled histogram) or isotype control antibody (Catalog # MAB002, open histo­gram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
IL‑22 R alpha 1 in HT‑29 Human Cell Line.
IL‑22 R alpha 1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line using Mouse Anti-Human IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB2770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-22 R alpha 1

IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL‑22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta  is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding IL‑22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22%‑25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.

  • References:
    1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
    2. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
    3. Boniface, K. et al. (2005) J. Immunol. 174:3695.
    4. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
    5. Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
    6. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
    7. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
    8. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
    9. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
    10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
    11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
    12. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
    13. Wolk, K. et al. (2004) Immunity 21:241.
    14. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
  • Long Name:
    Interleukin 22 Receptor
  • Entrez Gene IDs:
    58985 (Human); 230828 (Mouse)
  • Alternate Names:
    CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 R alpha 1; IL-22 receptor subunit alpha-1; IL22R alpha 1; IL22R; IL22R1; IL22RA1; IL-22Ra1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection
    Authors: P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader
    Mucosal Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes.
    Authors: Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier R
    J Neuroinflammation, 2015;12(0):119.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. The crosstalk between IL-22 signaling and miR-197 in human keratinocytes.
    Authors: Lerman, Galya, Sharon, Moran, Leibowitz-Amit, Raya, Sidi, Yechezke, Avni, Dror
    PLoS ONE, 2014;9(9):e107467.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  4. Interleukin-22 suppresses the growth of A498 renal cell carcinoma cells via regulation of STAT1 pathway.
    Authors: Zhang F, Shang D, Zhang Y, Tian Y
    PLoS ONE, 2011;6(5):e20382.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. TH17 cytokines induce human airway smooth muscle cell migration.
    Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
    J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Novel mechanism of MDA-7/IL-24 cancer-specific apoptosis through SARI induction.
    Authors: Dash R, Bhoopathi P, Das S, Sarkar S, Emdad L, Dasgupta S, Sarkar D, Fisher P
    Cancer Res, 0;74(2):563-74.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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