Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 305405
View all IL-22 R alpha 1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha 1
Pro18-Thr228
Accession # Q8N6P7

Specificity

Detects human IL‑22 R alpha 1 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) IL-10, rhIL-22BP or rhIL-20 R alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IL‑22 R alpha 1 Antibody

Detection of IL-22 Ra1 antibody in COLO 205 Human Cell Line antibody by Flow Cytometry.

Detection of IL‑22 R alpha 1 in COLO 205 Human Cell Line by Flow Cytometry.

COLO 205 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human IL-22 Ra1 Monoclonal Antibody (Catalog # MAB2770, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

IL-22 Ra1 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC).

IL‑22 R alpha 1 in HT‑29 Human Cell Line.

IL-22 Ra1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human IL-22 Ra1 Monoclonal Antibody (Catalog # MAB2770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of IL‑22 R alpha 1 in HT-29 cells by Flow Cytometry.

HT-29 cells were stained with Mouse Anti-Human IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB2770, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Detection of IL‑22 R alpha 1 in HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and HepG2 Human Hepatocellular Carcinoma Cell Line (Negative) Cells.

IL‑22 R alpha 1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive) and HepG2 human hepatocellular carcinoma cell line (negative) cells using Mouse Anti-Human IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB2770) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human IL-22R alpha 1 by Western Blot

Detection of Human IL-22R alpha 1 by Western Blot

miR-197 suppresses the expression of IL22RA1 by binding to its 3′UTR.a) miR-197 binding site on IL22RA1 3′UTR. b) HEK-293T cells were transfected with psi-CHECK2 vectors encoding luciferase (vector), luciferase fused to the IL22RA1-3′UTR (IL22RA1 wt 3′UTR), or luciferase fused to a mutated in miR-197 binding to the IL22RA1 3′UTR (IL22RA1 mut 3′UTR) together with 2 µg miR-197 expressing plasmid or 2 µg scramble RNA expressing plasmid. Cells transfected with only with vector lacking the IL22RA1 3′UTR was valued as 100%. The error bars are calculated as standard error of at least 6 independent experiments. c) Western blot (WB) analysis of IL22RA1 protein 48 h after transfection with 5/10 nM of scramble control pre-miR or with 5/10 nM of pre-miR-197. d) Densitometry analysis of 4 WBs analysis of IL22RA1 protein 48 h after transfection with 10 nM of scramble control RNA (scramb), or 10 nM pre-miR-197 *p = 0.0000818. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25208211), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL‑22 R alpha 1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: COLO 205 human colorectal adenocarcinoma cell line and HT‑29 human colon adenocarcinoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and Daudi Human Burkitt's Lymphoma Cell Line (Negative) Cells 

Reviewed Applications

Read 2 reviews rated 4 using MAB2770 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-22 R alpha 1

IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL‑22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta  is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding IL‑22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22%‑25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.

References

  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Boniface, K. et al. (2005) J. Immunol. 174:3695.
  4. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  5. Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
  6. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
  7. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
  8. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
  9. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
  10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
  11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
  12. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
  13. Wolk, K. et al. (2004) Immunity 21:241.
  14. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.

Long Name

Interleukin 22 Receptor

Alternate Names

CRF2-9, IL-22Ra1, IL-TIF-R1, IL22R alpha 1, IL22RA1

Entrez Gene IDs

58985 (Human); 230828 (Mouse)

Gene Symbol

IL22RA1

UniProt

Q8N6P7

Additional IL-22 R alpha 1 Products

Product Documents for Human IL‑22 R alpha 1 Antibody

Certificate of Analysis

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Product Specific Notices for Human IL‑22 R alpha 1 Antibody

For research use only

Citations for Human IL‑22 R alpha 1 Antibody

Customer Reviews for Human IL‑22 R alpha 1 Antibody (2)

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  • Human IL-22 R alpha 1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Epidermal cells and Dermal cells
    Species: Human
    Verified Customer | Posted 11/03/2021
    Human IL‑22 R alpha 1 Antibody MAB2770
  • Human IL-22 R alpha 1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 06/09/2020
    We used this antibody in an in-house ELISA along with pAb (AF2770) and protein standard (2770-LR) to quantify IL-22R alpha1 in human serum and plasma. This combination could not detect IL-22R alpha1 in our samples but generated a good standard curve.
    Human IL‑22 R alpha 1 Antibody MAB2770

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