< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine Human IL-28B/IFN-lambda 3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-28B levels in cell culture supernates, cell lysates, serum, EDTA plasma, and urine. It contains CHO cell-expressed recombinant human IL-28B and antibodies raised against the recombinant protein. Results obtained for naturally occurring human IL-28B showed linear curves that were parallel to the standard curves obtained using the Quantikine Human IL-28B Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-28B.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
The recovery of human IL-28B spiked to levels throughout the range of the assay in various matrices was evaluated.
Average % Recovery
Cell Culture Media (n=4)
Cell Lysis Buffer (n=4)
EDTA Plasma (n=4)
To assess the linearity of the assay, samples spiked with high concentrations of human IL-28B were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-28B/IFN-lambda 3
Human/mouse IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and human IL-29 (IFN-lambda 1) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and type I IFN family (15 - 19% aa sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28 shares 61%, 62%, and 52% aa identity with human IL-28A, IL-28B, and IL-29, respectively.