Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit

  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Urine (50 uL)
  • Sensitivity
    1.87 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Urine)
  • Specificity
    Natural and recombinant human IL-28B.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC222, Quantikine Immunoassay Control Set 885 Human IL-28B - Please Inquire
Product Summary
The Quantikine Human IL-28B/IFN-lambda 3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-28B levels in cell culture supernates, cell lysates, serum, EDTA plasma, and urine. It contains CHO cell-expressed recombinant human IL-28B and antibodies raised against the recombinant protein. Results obtained for naturally occurring human IL-28B showed linear curves that were parallel to the standard curves obtained using the Quantikine Human IL-28B Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-28B.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean90.727956098.6282532
Standard Deviation2.810.511.97.1519.936.1
CV%3.13.82.17.37.16.8

Recovery

The recovery of human IL-28B spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 113 83-122
Cell Lysis Buffer (n=4) 96 87-103
EDTA Plasma (n=4) 111 93-124
Serum (n=4) 111 96-124
Urine (n=4) 84 75-96
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human IL-28B were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-28B/IFN-lambda 3
Human/mouse IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and human IL-29 (IFN-lambda 1) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and type I IFN family (15 - 19% aa sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28 shares 61%, 62%, and 52% aa identity with human IL-28A, IL-28B, and IL-29, respectively.
    • Long Name
      Interleukin 28B
    • Entrez Gene IDs
      282617 (Human); 338374 (Mouse);
    • Alternate Names
      interleukin-28B; IFN-lambda 3; IL28B; IL-28B; IL28C; interferon, lambda 3;
    Related Research Areas
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 885 Human IL-28B

    Ctrl QC222
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    Supplemental ELISA Products
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    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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