Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
D28B00
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Human IL-28B/IFN-lambda 3 Standard Curve
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Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Urine (50 uL)
Sensitivity
1.87 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Urine)
Specificity
Natural and recombinant human IL-28B.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-28B/IFN-lambda 3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-28B levels in cell culture supernates, cell lysates, serum, EDTA plasma, and urine. It contains CHO cell-expressed recombinant human IL-28B and antibodies raised against the recombinant protein. Results obtained for naturally occurring human IL-28B showed linear curves that were parallel to the standard curves obtained using the Quantikine Human IL-28B Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-28B.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 90.7 279 560 98.6 282 532
Standard Deviation 2.8 10.5 11.9 7.15 19.9 36.1
CV% 3.1 3.8 2.1 7.3 7.1 6.8

Recovery

The recovery of human IL-28B spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 113 83-122
Cell Lysis Buffer (n=4) 96 87-103
EDTA Plasma (n=4) 111 93-124
Serum (n=4) 111 96-124
Urine (n=4) 84 75-96

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human IL-28B were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-28B/IFN-lambda 3 ELISA Linearity

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-28B/IFN-lambda 3

Human/mouse IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and human IL-29 (IFN-lambda 1) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and type I IFN family (15 - 19% aa sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28 shares 61%, 62%, and 52% aa identity with human IL-28A, IL-28B, and IL-29, respectively.

Long Name:
Interleukin 28B
Entrez Gene IDs:
282617 (Human); 338374 (Mouse)
Alternate Names:
interleukin-28B; IFN-lambda 3; IL28B; IL-28B; IL28C; interferon, lambda 3
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

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